caa a22 4500 477110177 CHVBK 20180405111606.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF00012053 doi (NATIONALLICENCE)springer-10.1007/BF00012053 Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots [Elektronische Daten] [S. Wright, M. Franke-Snyder, J. Morton, A. Upadhyaya] Material on the surface of hyphal walls of arbuscular mycorrhizal fungi (AMF) during active colonization of plant roots was detected by a monoclonal antibody. Pot-cultured isolates of Glomus, Acaulospora, Gigaspora, Scutellospora, and Entrophospora had immunofluorescent material (IM) on younger, thinner, intact hyphae, but IM was scant to absent on thicker, melanized or lysing hyphae. Colonization of corn (Zea mays L.), Sudangrass (Sorghum sudanense (Piper) Staph.) or red clover (Trifolium pratense L.) was examined during 5 months of plant growth by removing cores and performing an indirect immunoassay on roots with attached hyphae. Fresh spores of some Glomus spp. had IM on the outer layer of the spore wall. Abundant IM was seen on root hairs of plants colonized by some isolates, and some IM was detected on root surfaces of all plants examined even during early colonization. After cultures were dried, hyphae, roots and spores had little to no IM. Uninoculated control roots had very rare, small patches of IM. An immunoreactive protein was extracted from hyphae of Gigaspora and Glomus isolates by using 20mM citrate (pH 7.0) at 121°C for 90 min. Gel electrophoresis profiles indicated that all isolates tested had the same banding patterns. Lectin-binding of extracted protein is suggestive of a glycoprotein. The immunofluorescence assay can be used to examine root sections for active colonization by AMF, and the potential use of the protein to quantify AMF activity in soil is discussed. Kluwer Academic Publishers, 1996 fungal protein nationallicence heat-stable protein nationallicence Glomales nationallicence vesicular arbuscular mycorrhizal fungi nationallicence Wright S. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut Franke-Snyder M. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut Morton J. Division of Plant and Soil Sciences, West Virginia University, 26506-6057, Morgantown, WV, USA aut Upadhyaya A. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 181/2(1996-04-01), 193-203 0032-079X 181:2<193 1996 181 11104 https://doi.org/10.1007/BF00012053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00012053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wright S. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Franke-Snyder M. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Morton J. Division of Plant and Soil Sciences, West Virginia University, 26506-6057, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Upadhyaya A. Soil Microbial Systems Laboratory, United States Department of Agriculture-Agricultural Research Service, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 181/2(1996-04-01), 193-203 0032-079X 181:2<193 1996 181 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer