caa a22 4500 477110290 CHVBK 20180405111606.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00011160 doi (NATIONALLICENCE)springer-10.1007/BF00011160 Determination of chitin in fungi and mycorrhizal roots by an improved HPLC analysis of glucosamine [Elektronische Daten] [Alf Ekblad, Torgny Näsholm] A method to measure chitin content in fungi and ectomycorrhizal roots with high-performance liquid chromatography (HPLC) was developed. Measurements of fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine were made on acid hydrolysates of pure chitin, chitin-root mixtures and fungal-root mixtures. The method was applied on 5 isolates of ectomycorrhizal fungi, and ectomycorrhizal and non-mycorrhizal Pinus sylvestris roots. Interference from amino acids was removed by pre-treatment of samples with 0.2 N NaOH. This pre-treatment did not reduce the recovery of chitin, nor did plant material affect the recovery of chitin. The HPLC method was compared with a colorimetric chitin-method by measurements on root-fungal mixtures, with known fungal content. The HPLC method gave estimates of fungal biomass which were equal to the expected while the colorimetric method showed values significantly (p<0.001) lower than the expected. The present chitin method offers a sensitive and specific tool for the quantification of chitin in fungi and in ectomycorrhizal roots. Kluwer Academic Publishers, 1996 chitin nationallicence ectomycorrhizal fungi nationallicence glucosamine nationallicence high-performance liquid chromatography nationallicence Ekblad Alf Department of Plant Physiology, Umeå University, S-901 87, Umeå, Sweden aut Näsholm Torgny Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 178/1(1996-01-01), 29-35 0032-079X 178:1<29 1996 178 11104 https://doi.org/10.1007/BF00011160 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00011160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ekblad Alf Department of Plant Physiology, Umeå University, S-901 87, Umeå, Sweden aut NATIONALLICENCE 700 1- Näsholm Torgny Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 178/1(1996-01-01), 29-35 0032-079X 178:1<29 1996 178 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer