caa a22 4500 47711038X CHVBK 20180405111607.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00011165 doi (NATIONALLICENCE)springer-10.1007/BF00011165 Glycolytic gene expression in amphibious Acorus calamus L. under natural conditions [Elektronische Daten] [Marcel Bucher, Roland Brändle, Cris Kuhlemeier] Acorus calamus L is an amphibious plant, which is exposed to periods of flooding and consequently hypoxic conditions as a part of its natural life cycle. Previous experiments under laboratory conditions have shown that the plant can survive for two months in the complete absence of oxygen, and that during this period the expression of genes encoding the glycolytic enzymes fructose-1,6-bisphosphate aldolase (ALD), pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) is induced in leaves and rhizomes (Bucher and Kuhlemeier, 1993). Here we studied the expression of ALD and ADH through two years in the natural habitat of A. calamus. Under natural conditions roots and rhizomes were always submerged but newly grown leaves emerged in spring; in autumn the leaves senesced and the whole plant was submerged again. High Ald and Adh mRNA levels in leaf and rhizome were found only in winter when the leaves were entirely submerged. Upon leaf emergence in spring the mRNA levels rapidly declined. Under controlled experimental conditions expression of Ald and Adh was not induced by low temperature. The combination of laboratory and field experiments supports the hypothesis that oxygen deprivation rather than low temperature is a major regulator of glycolytic gene expression in A. calamus. The possible role of other environmental factors is also discussed. Kluwer Academic Publishers, 1996 cold acclimation nationallicence ecophysiology nationallicence fermentation nationallicence flooding nationallicence gene expression nationallicence ADH : alcohol dehydrogenase nationallicence Adh : gene encoding ADH nationallicence ALD : cytoplasmic fructose-1,6-bisphosphate aldolase nationallicence Ald : gene encoding ALD nationallicence PDC : pyruvate decarboxylase nationallicence Pdc : gene encoding PDC nationallicence Bucher Marcel Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut Brändle Roland Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut Kuhlemeier Cris Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 178/1(1996-01-01), 75-82 0032-079X 178:1<75 1996 178 11104 https://doi.org/10.1007/BF00011165 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00011165 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bucher Marcel Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut NATIONALLICENCE 700 1- Brändle Roland Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut NATIONALLICENCE 700 1- Kuhlemeier Cris Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 178/1(1996-01-01), 75-82 0032-079X 178:1<75 1996 178 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer