caa a22 4500 477110509 CHVBK 20180405111607.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00011580 doi (NATIONALLICENCE)springer-10.1007/BF00011580 The role of nicotianamine synthase in response to Fe nutrition status in Gramineae [Elektronische Daten] [Kyoko Higuchi, Kenji Kanazawa, Naoko-Kishi Nishizawa, Satoshi Mori] Nicotianamine is an intermediate for the biosynthesis of mugineic acid-family phytosiderophores (MAs) in the Gramineae and a key substance for iron metabolism in dicots. Nicotianamine synthase catalyzes the formation of nicotianamine from S-adenosylmethionine. Nicotianamine synthase activity was induced in barley roots at the 3rd day after withholding Fe supply and declined within one day followmg the supply of Fe3+-epihydroxymugineic acid. The induction of nicotianamine synthase activity by Fe-deficiency was observed also in sorghum, maize, and rye, and the level of nicotianamine synthase activity was highly associated with the MAs secreted among graminaceous plant tested. Therefore, the nicotianamine synthase gene may be a suitable candidate for making a transgenic plant tolerant to Fe-deficiency. Kluwer Academic Publishers, 1996 Fe-deficiency nationallicence graminaceous plant nationallicence mugineic acids nationallicence phytosiderophore nationallicence nicotianamine synthase nationallicence p-APMSF : (p-amidinophenyl) methanesulfonylfluoride hydrochloride nationallicence NA : nicotianamine nationallicence DMA : 2′-deoxymugineic acid nationallicence E-64 : trans-epoxysuccinyl-leucylamido-(4-guanidino) butane nationallicence epiHMA : 3-epihydroxymugineic acid nationallicence MAs : mugineic acid-family phytosiderophores which include deoxymugineic acid, mugineic acid, hydroxymugineic acid, epihydroxymugineic acid and avenic acid nationallicence PVP : polyvinylpyrrolidone nationallicence SAM : S-adenosylmethionine nationallicence Higuchi Kyoko Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut Kanazawa Kenji Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut Nishizawa Naoko-Kishi Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut Mori Satoshi Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 178/2(1996-01-01), 171-177 0032-079X 178:2<171 1996 178 11104 https://doi.org/10.1007/BF00011580 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00011580 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Higuchi Kyoko Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Kanazawa Kenji Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishizawa Naoko-Kishi Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Mori Satoshi Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, Yayoi 1-1 Bunkyo-ku, 113, Tokyo, Japan aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl// 178/2(1996-01-01), 171-177 0032-079X 178:2<171 1996 178 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer