caa a22 4500 477110673 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035049 doi (NATIONALLICENCE)springer-10.1007/BF00035049 Advances in the positional cloning of nodulation genes in soybean [Elektronische Daten] [Gustavo Caetano-Anollés, Peter Gresshoff] The positional cloning of genes involved in plant control of infection and nodule formation in legumes requires the development of improved tools for the analysis of large genomes and cloning of high molecular weight DNA. We have used bulked segregant analysis (BSA) and DNA amplification fingerprinting (DAF) with arbitrary oligonucleotide primers to detect polymorphisms linked to genes important to nodulation in soybean (Glycine max L. Merrill). Three loci controlling legume nodulation (nts-1, rj1 and rj6) and one involved in early nodule development (enod2) were studied. Wild-type Bragg and EMS-induced mutants defective in autoregulation (nts382) or nodulation (nod49 and nod139) were crossed with G. soja (Sieb. and Zucc.), for the analysis of segregants in F2 and F3 populations. DNA pools from wild-type and mutant individuals, or DNA pools based on RFLP pattern, were screened with sets of structured (mini-hairpin) and unstructured primers, identifying polymorphic DNA. DAF with mini-hairpin primers, template endonuclease cleaved DAF (tecMAAP), and arbitrary signatures from amplification profiles (ASAP) were especially successful in detecting linked polymorphisms. Putative markers were confirmed by individual analysis of segregants, and some of them converted into sequence-characterized amplified regions (SCAR). These markers will be used for high density mapping of the relevant genomic regions and as landmarks for linking cloned soybean DNA from yeast and bacterial artificial chromosome (YAC and BAC) libraries. At present, the partial YAC library (avg. insert size = 200 kb) represents about 20% of the soybean genome. YAC endclones were isolated using vectorette PCR, and consisted of unique or repeated DNA. Together with YAC-specific signatures generated with mini-hairpin primers, they will be used in the construction of contigs for positional cloning. Kluwer Academic Publishers, 1996 arbitrary primers nationallicence BAC nationallicence BSA nationallicence DAF nationallicence genetic markers nationallicence nodulation nationallicence positional cloning nationallicence symbiosis genes nationallicence soybean nationallicence YAC nationallicence Caetano-Anollés Gustavo Plant Molecular Genetics, Institute of Agriculture, 37901, Knoxville, TN, USA aut Gresshoff Peter Plant Molecular Genetics, Institute of Agriculture, 37901, Knoxville, TN, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 1-7 0032-079X 186:1<1 1996 186 11104 https://doi.org/10.1007/BF00035049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caetano-Anollés Gustavo Plant Molecular Genetics, Institute of Agriculture, 37901, Knoxville, TN, USA aut NATIONALLICENCE 700 1- Gresshoff Peter Plant Molecular Genetics, Institute of Agriculture, 37901, Knoxville, TN, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 1-7 0032-079X 186:1<1 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer