caa a22 4500 47711072X CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035062 doi (NATIONALLICENCE)springer-10.1007/BF00035062 Analysis of the two nodulins, sucrose synthase and ENOD2, in transgenic Lotus plants [Elektronische Daten] [L. Skøt, F. Minchin, E. Timms, M. Fortune, K. Webb, A. Gordon] A partial sucrose synthase clone was isolated from a nodule cDNA library of Lotus japonicus, which had strong homology to sucrose synthase genes from Vicia faba and other plant species. The transcript was 2.7 kb in size and expression was enhanced in nodules compared to roots of L. japonicus. Two overlapping genomic clones containing sucrose synthase sequences were isolated from L. japonicus with a view to mapping and analysing the promoter of this gene. The early nodulin ENOD2 is a (hydroxy)proline-rich cell wall protein, which is expressed in the inner cortex surrounding the infected region of root nodules. In order to see if this nodulin is involved in the operation of the oxygen diffusion barrier, L. corniculatus was transformed with antisense ENOD2 gene constructs derived from the promoter and coding sequence of soybean. Two of the 20 antisense plant lines had significantly lower dry weight and nitrogenase activity as well as a different O2 profile through the nodules when compared to control plant lines, but there was no difference in ENOD2 transcriptional activity between the control and antisense plants. Possible reasons for this observation are discussed. Kluwer Academic Publishers, 1996 antisense RNA nationallicence ENOD2 nationallicence Lotus nationallicence nodulin nationallicence oxygen diffusion barrier nationallicence sucrose synthase nationallicence kDa : kilodalton nationallicence kb : kilobases nationallicence CAT : chloramphenicol acetyl transferase nationallicence CaMV : Cauliflower Mosaic Virus nationallicence Nos : Nopaline Synthase nationallicence Skøt L. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Minchin F. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Timms E. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Fortune M. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Webb K. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Gordon A. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 99-106 0032-079X 186:1<99 1996 186 11104 https://doi.org/10.1007/BF00035062 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035062 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skøt L. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 700 1- Minchin F. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 700 1- Timms E. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 700 1- Fortune M. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 700 1- Webb K. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 700 1- Gordon A. Institute of Grassland and Environmental Research, Plas Gogerddan, SY23 3EB, Aberystwyth, Dyfed, Wales, UK aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 99-106 0032-079X 186:1<99 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer