caa a22 4500 477110754 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035050 doi (NATIONALLICENCE)springer-10.1007/BF00035050 Molecular analysis of actinorhizal symbiotic systems: Progress to date [Elektronische Daten] [Beth Mullin, Svetlana Dobritsa] The application of molecular tools to questions related to the genetics, ecology and evolution of actinorhizal symbiotic systems has been especially fruitful during the past two years. Host plant phylogenies based on molecular data have revealed markedly different relationships among host plants than have previously been suspected and have contributed to the development of new hypotheses on the origin and evolution of actinorhizal symbiotic systems. Molecular analyses of host plant gene expression in developing nodules have confirmed the occurrence of nodulin proteins and in situ hybridization techniques have been successfully adapted to permit the study of the spatial and temporal patterns of gene expression within actinorhizal nodules. The use of heterologous probes in combination with nucleotide sequence analysis have allowed a number of nif genes to be mapped on the Frankia chromosome which will ultimately contribute to the development of hypotheses related to nif gene regulation in Frankia. The use of both 16S and 23S rDNA nucleotide sequences has allowed the construction of phylogenetic trees that can be tested for congruence with symbiotic characters. In addition the development of Frankia-specific gene probes and amplification primers have contributed to studies on the genetic diversity and distribution of Frankia in the soil. Kluwer Academic Publishers, 1996 actinorhizal symbiosis nationallicence ecology nationallicence Frankia nationallicence gene expression nationallicence molecular analysis nationallicence Mullin Beth Department of Botany, The University of Tennessee, 37996, Knoxville, TN, USA aut Dobritsa Svetlana Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142292, Pushchino, Moscow Region, Russia aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 9-20 0032-079X 186:1<9 1996 186 11104 https://doi.org/10.1007/BF00035050 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035050 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mullin Beth Department of Botany, The University of Tennessee, 37996, Knoxville, TN, USA aut NATIONALLICENCE 700 1- Dobritsa Svetlana Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142292, Pushchino, Moscow Region, Russia aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 9-20 0032-079X 186:1<9 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer