caa a22 4500 477110762 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035064 doi (NATIONALLICENCE)springer-10.1007/BF00035064 Presence of unique repeated insertion sequences in nodulation genes of Rhizobium ‘hedysari' [Elektronische Daten] [F. Meneghetti, S. Alberghini, E. Tola, A. Giacomini, F. Ollero, A. Squartini, M. Nuti] Cloning and sequencing of DNA from a symbiotic large plasmid in Rhizobium ‘hedysari' strain IS 123 required for its nodulation of the mediterranean legume crop Hedysarum coronarium (sulla) and complementation studies of nod- mutant derivatives led to the characterization of a 30-kb region containing common and host-specific nod genes. This DNA region also contained at least six copies of a novel insertion sequence-like structure, some of which appeared to have suffered deletions. This 0.8 kb novel element carries two 17-bp flanking inverted repeats and an open reading frame showing homology with a transposase from Staphylococcus aureus. Hybridization studies revealed that several strains of Rhizobium ‘hedysari' carry this element in various copy number. The six copies in strain IS 123 appear clustered specifically within the pSym nod region. The significance of this IS element in rhizobia and its possible use as a probe for taxonomic and phylogenetic studies of Rhizobiaceae is addressed. Kluwer Academic Publishers, 1996 Hedysarum coronarium nationallicence IS element nationallicence nodulation genes nationallicence Rhizobium ‘hedysari' nationallicence Meneghetti F. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Alberghini S. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Tola E. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Giacomini A. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Ollero F. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Squartini A. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Nuti M. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 113-120 0032-079X 186:1<113 1996 186 11104 https://doi.org/10.1007/BF00035064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meneghetti F. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Alberghini S. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Tola E. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Giacomini A. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Ollero F. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Squartini A. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 700 1- Nuti M. Dipartimento di Biotecnologie Agrarie, Universitá di Padova, via Gradenigo 6, 35131, Padova, Italy aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 113-120 0032-079X 186:1<113 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer