caa a22 4500 477110797 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035067 doi (NATIONALLICENCE)springer-10.1007/BF00035067 A diazotrophic bacterial endophyte isolated from stems of Zea mays L. and Zea luxurians Iltis and Doebley [Elektronische Daten] [James Palus, James Borneman, Paul Ludden, Eric Triplett] The apoplastic fluids of field-grown Zea mays and Zea luxurians plants were isolated from surface sterilized stem tissue by centrifugation and spread on agar plates containing a nitrogen-free, defined medium. The predominant bacterium isolated from these plates was characterized further. The ability of this bacterium to fix nitrogen was confirmed by its ability to grow on a semi-solid, nitrogen-free medium and reduce 15N2 to 15NH3 and acetylene to ethylene. Protions of the nifH and 16S rRNA genes from this organism were amplified by PCR and sequenced. The nifH gene, which codes for dinitrogenase reductase, from this organism is closely related to nifH from Klebsiella pneumoniae. Similarly, the 16S rRNA gene sequences and carbon utilization tests grouped it closely with K. pneumoniae. Based an these data, the isolates from Z. mays and Z. luxurians are tentatively classified as Klebsiella spp. (Zea). The ability of this bacterium to contribute to the nitrogen economy of the corn plant is unknown. Kluwer Academic Publishers, 1996 diazotrophs nationallicence endophytes nationallicence Klebsiella nationallicence sweet corn Zea nationallicence Palus James Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut Borneman James Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut Ludden Paul Department of Biochemistry, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut Triplett Eric Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 135-142 0032-079X 186:1<135 1996 186 11104 https://doi.org/10.1007/BF00035067 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035067 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palus James Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut NATIONALLICENCE 700 1- Borneman James Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut NATIONALLICENCE 700 1- Ludden Paul Department of Biochemistry, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut NATIONALLICENCE 700 1- Triplett Eric Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, 53706-1597, Madison, WI, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 135-142 0032-079X 186:1<135 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer