caa a22 4500 477110827 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035063 doi (NATIONALLICENCE)springer-10.1007/BF00035063 Hopanoid lipids in Bradyrhizobium and other plant-associated bacteria and cloning of the Bradyrhizobium japonicum squalene-hopene cyclase gene [Elektronische Daten] [E. Kannenberg, M. Perzl, P. Müller, T. Härtner, K. Poralla] Hopanoid lipids have been discovered recently in a number of nitrogen-fixing soil bacteria and in Bradyrhizobium bacteria which fix nitrogen in association with legume plants. We report here an investigation of the hopanoid content in an additional number of soil bacteria capable of living in close association with plants. Of the strains investigated, hopanoids were discovered in phototrophic, nitrogen-fixing bacteria and in an extended number of Bradyrhizobium strains. Strains in which hopanoids so far have not been found belong to the following genera: Rhizobium, Sinorhizobium, Phyllobacterium, Agrobacterium, and Azoarcus. To address the function of hopanoids in Bradyrhizobium, we cloned the gene coding for a key enzyme of hopanoid biosynthesis, the squalene-hopene cyclase, and expressed the gene in E. coli. The recombinant enzyme catalyzed in vitro the cyclization of squalene to hopanoid derivatives. Kluwer Academic Publishers, 1996 Bradyrhizobium nationallicence hopanoids nationallicence nitrogen fixation nationallicence plant-microbe interaction nationallicence Rhizobium nationallicence squalene-hopene cyclase nationallicence SHC : squalene-hopene cyclase nationallicence shc : squalene-hopene cyclase gene nationallicence Kannenberg E. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Perzl M. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Müller P. Fachbereich Biologie-Botanik, Universität Marburg, D-35043, Marburg, Germany aut Härtner T. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Poralla K. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 107-112 0032-079X 186:1<107 1996 186 11104 https://doi.org/10.1007/BF00035063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kannenberg E. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Perzl M. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Müller P. Fachbereich Biologie-Botanik, Universität Marburg, D-35043, Marburg, Germany aut NATIONALLICENCE 700 1- Härtner T. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 700 1- Poralla K. Mikrobiologie/Biotechnologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 107-112 0032-079X 186:1<107 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer