caa a22 4500 477110835 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035056 doi (NATIONALLICENCE)springer-10.1007/BF00035056 Assimilation of nod gene inducer 14C-naringenin and the incorporation of labelled carbon atoms into the acyl side chain of a host-specific Nod factor produced by Rhizobium leguminosarum bv. viciae [Elektronische Daten] [J. Rao, J. Cooper, E. Everaert, L. De Cooman] The fate of 14C-naringenin during its specific activation of nod genes in Rhizobium leguminosarum bv. viciae was examined. After incubation with either strain RBL5560 or its pSym-cured derivative in a medium supplemented with 14C-naringenin at nod gene-inducing concentrations of 2 nM (ca. 12.5 kBq) plus cold acetate (0.5 μM), a radiocarbon inventory for the cells and supernatant extracts was obtained. The level of 14C-label incorporation was also determined in the fractionated cellular components. Using 14C-acetate at 0.5 μM (1036 kBq) and cold naringenin (2 nM) in incubations with strain RBL5560 as a separate treatment, the Nod metabolites were detected by thin layer and high performance liquid chromatographic methods and the data provided the basis for identification of the Nod factors from the supernatant obtained from 14C-naringenin treatments. Subsequent radio-biochemical and chemical analyses revealed that RBL5560 cells assimilated 14C-naringenin during the activation of nod genes. Our analysis also showed that labelled carbon atoms from the 14C-naringenin were incorporated into the acyl moiety of a lipo-oligosaccharide Nod factor, NodRlv IV, present in the culture supernatants of RBL5560. The pSym-cured derivative failed to synthesize any Nod metabolites in a 14C-naringenin supplemented medium. The tracing of flavonoid-derived carbon atoms to the acyl chain of a host-specific Nod factor, a moiety that defines host specificity for this Rhizobium, adds a new dimension to the signalling function of flavonoids in legume-Rhizobium interactions. Kluwer Academic Publishers, 1996 14C-naringenin assimilation nationallicence Nod factor synthesis nationallicence Rhizobium nod gene-inducer nationallicence Ac : acyl chain nationallicence ca : calculated approximately nationallicence dpm : disintegrations per minute nationallicence HPLC : High Performance Liquid Chromatography nationallicence pSym : symbiotic plasmid nationallicence R. : Rhizobium nationallicence TLC : Thin Layer Chromatography nationallicence Rao J. Department of Applied Plant Science, The Queen's University of Belfast, BT9 5PX, Belfast, Northern Ireland, UK aut Cooper J. Department of Applied Plant Science, The Queen's University of Belfast, BT9 5PX, Belfast, Northern Ireland, UK aut Everaert E. Laboratory of Plant Biochemistry, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut De Cooman L. Laboratory of Plant Biochemistry, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 63-67 0032-079X 186:1<63 1996 186 11104 https://doi.org/10.1007/BF00035056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rao J. Department of Applied Plant Science, The Queen's University of Belfast, BT9 5PX, Belfast, Northern Ireland, UK aut NATIONALLICENCE 700 1- Cooper J. Department of Applied Plant Science, The Queen's University of Belfast, BT9 5PX, Belfast, Northern Ireland, UK aut NATIONALLICENCE 700 1- Everaert E. Laboratory of Plant Biochemistry, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 700 1- De Cooman L. Laboratory of Plant Biochemistry, Rijksuniversiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 63-67 0032-079X 186:1<63 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer