caa a22 4500 477110843 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035059 doi (NATIONALLICENCE)springer-10.1007/BF00035059 Detection of genetic variation in Bradyrhizobium japonicum USDA 110 variants using DNA fingerprints generated with GC rich arbitrary PCR primers [Elektronische Daten] [James Mathis, David McMillin] Bradyrhizobium japonicum USDA 110 has been shown to contain several genetically similar naturally occurring colony morphology variants. These variants differ in symbiotic nitrogen fixation ability and in the utilization of various carbon substrates. They have been shown to share extensive DNA homology and appear to be derived from a common ancestor. Despite these similarities certain B. japonicum USDA 110 variants have been shown to be devoid of symbiotic nitrogen fixation. One of these variants (L2-110), however, was recently shown to possess significant levels of explanta nitrogen fixation and to synthesize functional dinitrogenase enzyme within bacteroids. In an effort to identify genetic markers which could explain differences in symbiotic nitrogen fixation between B. japonicum variants, DNA fingerprints were generated by PCR using arbitrary primers. Two of these primers with GC rich sequences were able to differentiate between B. japonicum USDA 110 variants I-110, L2-110, and MN-110. Unique markers have now been identified which could be examined further to determine if they explain the differences in symbiotic nitrogen fixation between USDA 110 variants. Kluwer Academic Publishers, 1996 Bradyrhizobium japonicum colony morphology variants nationallicence DNA fingerprints nationallicence GC rich arbitrary primers RAPD-PCR nationallicence Mathis James Department of Biology, State University of West Georgia, 30118, Carrollton, GA, USA aut McMillin David Department of Biology, State University of West Georgia, 30118, Carrollton, GA, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 81-85 0032-079X 186:1<81 1996 186 11104 https://doi.org/10.1007/BF00035059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mathis James Department of Biology, State University of West Georgia, 30118, Carrollton, GA, USA aut NATIONALLICENCE 700 1- McMillin David Department of Biology, State University of West Georgia, 30118, Carrollton, GA, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 81-85 0032-079X 186:1<81 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer