caa a22 4500 477110851 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035061 doi (NATIONALLICENCE)springer-10.1007/BF00035061 Subnanomolar concentrations of membrane chitolipooligosaccharides from Rhizobium leguminosarum biovar trifolii are fully capable of eliciting symbiosis-related responses on white clover [Elektronische Daten] [Guy Orgambide, Saleela Philip-Hollingsworth, Pedro Mateos, Rawle Hollingsworth, Frank Dazzo] Axenic seedling bioassays were performed on white clover, vetch, and alfalfa to assess the variety and dose responses of biological activities exhibited by membrane chitolipooligosaccharides (CLOSs) from wild type Rhizobium leguminosarum bv. trifolii ANU843. Subnanomolar concentrations of CLOSs induced deformation of root hairs (Had) and increased the number of foci of cortical cell divisions (Ccd) in white clover, some of which developed into nodule meristems. In contrast, ANU843 CLOSs were unable to induce Had in alfalfa and required a 104-fold higher threshold concentration to induce this response in vetch. Also, ANU843 CLOSs were not mitogenic on either of these non-host legumes. In addition, CLOS action also increased chitinase activity in white clover root exudate. Thus, the membrane CLOSs from wild type R. leguminosarum bv. trifolii are fully capable of eliciting various symbiosis-related responses in white clover in the same concentration range as extracellular CLOSs of other rhizobia on their respective legume hosts. These results and our earlier studies indicate that membrane CLOSs represent one of many different classes of bioactive metabolites made by R. leguminosarum bv. trifolii which elicit more intense symbiosis-related responses in white clover than in other legumes. Therefore, CLOSs evidently play an important role in symbiotic development, but they may not be the sole determinant of host-range in the Rhizobium-clover symbiosis. Kluwer Academic Publishers, 1996 chitolipooligosaccharide nationallicence Rhizobium-Trifolium repens symbiosis nationallicence root hair nationallicence Ccd : cortical cell division nationallicence CLOS : chitolipooligosaccharide nationallicence Had : root hair deformation nationallicence Orgambide Guy Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut Philip-Hollingsworth Saleela Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut Mateos Pedro Departamento de Microbiologia y Genetica, Facultad de Farmacia, Universidad de Salamanca, 37007, Salamanca, Spain aut Hollingsworth Rawle Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA aut Dazzo Frank Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 93-98 0032-079X 186:1<93 1996 186 11104 https://doi.org/10.1007/BF00035061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orgambide Guy Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Philip-Hollingsworth Saleela Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Mateos Pedro Departamento de Microbiologia y Genetica, Facultad de Farmacia, Universidad de Salamanca, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Hollingsworth Rawle Department of Biochemistry, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Dazzo Frank Department of Microbiology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 93-98 0032-079X 186:1<93 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer