caa a22 4500 477110886 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035066 doi (NATIONALLICENCE)springer-10.1007/BF00035066 Phage susceptibility and plasmid profile analysis of Sinorhizobium fredii [Elektronische Daten] [F. Hashem, L. Kuykendall, S. Udell, P. Thomas] This is the first report identifying bacteriophages and documenting megaplasmids of Sinorhizobium fredii. Plasmid DNA content and bacteriophage typing of eighteen strains of S. fredii were determined. S. fredii strains fell into ten plasmid profile groups containing 1 to 6 plasmids, some evidently larger than 1000 MDa. Twenty-three S. fredii lytic phages were isolated from soil, and they lysed six different S. fredii strains. The host range and plaque morphology of these phages were studied. Susceptibility to S. fredii phages was examined for S. meliloti; Rhizobium leguminosarum bvs. viceae, trifolii and Phaseoli; R. loti; Bradyrhizobium japonicum; B. elkanii and Bradyrhizobium sp. (Arachis). Several phages that originally lysed S. fredii strain USDA 206 also lysed strains of all three S. fredii serogroups described originally by Sadowsky et al. Phages that infected S. fredii strains USDA 191 and USDA 257 were highly specific and lysed only serogroup 193 strains. S. meliloti strains L5-30 and USDA 1005 were lysed by three of the phages that lysed S. fredii strain USDA 217. No other Rhizobium or Bradyrhizobium strain tested was susceptible to lysis by any of the S. fredii phages. The present investigation indicates that phage susceptibility in conjunction with plasmid profile analysis may provide a rapid method for identification and characterization of strains of S. fredii. Kluwer Academic Publishers, 1996 host range nationallicence megaplasmid nationallicence phage typing nationallicence rhizobiophage nationallicence Rhizobium fredii nationallicence Hashem F. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut Kuykendall L. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut Udell S. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut Thomas P. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 127-134 0032-079X 186:1<127 1996 186 11104 https://doi.org/10.1007/BF00035066 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035066 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashem F. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Kuykendall L. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Udell S. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Thomas P. Soybean and Alfalfa Research Laboratory, USDA/ARS, 10300 Baltimore Avenue, Building 011, HH-19, 20705-2350, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 127-134 0032-079X 186:1<127 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer