caa a22 4500 477110894 CHVBK 20180405111608.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00035070 doi (NATIONALLICENCE)springer-10.1007/BF00035070 Lipopolysaccharide core components of Rhizobium etli reacting with a panel of monoclonal antibodies [Elektronische Daten] [Elmar Kannenberg, L. Forsberg, Russell Carlson] Monoclonal antibodies that react with Rhizobium leguminosarum lipopolysaccharide core antigens (LPS-2) have been used to investigate LPS-2 structure in Rhizobium etli. The panel of antibodies (JIM 32 - JIM 35, JIM 37, JIM 38) specific for LPS-2 of R. leguminosarum strain 3841 and its core components displays similar reactivities towards isolated LPS-2 from R. etli CE109 (a mutant of wild-type strain R. etli CE3 that displays LPS-2 as its main LPS form on the cell surface). This result suggests the antibodies bind to similar epitopes on both strains and, hence, that R. leguminosarum and R. etli have very similar LPS core and lipid A antigen structures. More detailed analysis of the antibody binding sites with isolated LPS-2 and lipid A from R. etli suggests that some of the antibodies (JIM 32, 33, 34, and MASM-I) bind some part of the core oligosaccharides, while others (JIM 35 and JIM 38) involve lipid A. These antibodies have already proven useful in the biochemical analysis of the LPS antigen forms. For example, the loss of reactivity of certain LPS forms with antibody JIM 37 has led to the discovery of a hitherto unnoticed form of the LPS antigen in a precipitate formed during the phenol/water extraction procedure. This new form reacts with the JIM 37 antibody. Furthermore, the positive reaction of some of the antibodies with only sonicated wild-type R. etli cells suggests that either an effective way of masking the display of core antigens on whole bacterial cells is occurring or that core forms of the LPSs are never displayed on the surface of the bacterial cells. Either possibility, once confirmed, could be important for our picture of the Rhizobium cell surface and could also have some bearing on symbiotic nodule infection and development. Kluwer Academic Publishers, 1996 lipopolysaccharide nationallicence plant-microbe interaction nationallicence Rhizobium nationallicence symbiosis nationallicence LPS : lipopolysaccharide nationallicence Kannenberg Elmar Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut Forsberg L. Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut Carlson Russell Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 186/1(1996-09-01), 161-166 0032-079X 186:1<161 1996 186 11104 https://doi.org/10.1007/BF00035070 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00035070 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kannenberg Elmar Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut NATIONALLICENCE 700 1- Forsberg L. Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut NATIONALLICENCE 700 1- Carlson Russell Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl/ 186/1(1996-09-01), 161-166 0032-079X 186:1<161 1996 186 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer