caa a22 4500 477111181 CHVBK 20180405111609.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00011645 doi (NATIONALLICENCE)springer-10.1007/BF00011645 ESPAS — An advanced phytotron for measuring carbon dynamics in a whole plant-soil system [Elektronische Daten] [A. Gorissen, P. Kuikman, J. van Ginkel, H. van de Beek, A. Jansen] The use of carbon isotopes as tracers is essential for measuring carbon flows in an intact whole plant-soil system. Here, we describe and Experimental Soil Plant Atmosphere System (ESPAS) to perform pulse-labelling and steady-state labelling experiments with 13CO2 and 14CO2. The ESPAS facility is an environmental research tool that is used to measure the carbon fluxes from the atmosphere to the roots and into the soil and the microbial biomass and to study decomposition of plant residues and soil organic matter. The influence of environmental conditions in the atmosphere or in soil on the carbon allocation and turnover in the plant-soil ecosystem can be quantified. The design and the technical description of the phytotrons is presented and evidence is provided that the phytotrons are equivalent. For this purpose, Triticum aestivum plants were cultivated in the phytotrons for 39 days and shoot growth, root growth and water use were compared. No significant differences were observed for plant growth and water use. As an example of the practical application of the equipment, an experiment with elevated atmospheric CO2 is presented. Data are given on the uptake of 14C under ambient (350 μL L−1) and elevated (700 μL L−1CO2) in Lolium perenne and Festuca arundinacea and the distribution of 14C among different plant-soil compartments i.e. shoot, root, root-soil respiration, and soil. We conclude that these phytotrons yield detailed information on gross carbon flows in a whole plant-soil system that can not be obtained without sensitive carbon tracers. Such data are important for proper calibration of simulation models on soil organic matter. Kluwer Academic Publishers, 1996 carbon allocation nationallicence phytotron nationallicence pulse-labelling nationallicence steady-state labelling nationallicence 13C nationallicence 14C nationallicence Gorissen A. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut Kuikman P. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut van Ginkel J. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut van de Beek H. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut Jansen A. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 179/1(1996-02-01), 81-87 0032-079X 179:1<81 1996 179 11104 https://doi.org/10.1007/BF00011645 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00011645 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gorissen A. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kuikman P. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van Ginkel J. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van de Beek H. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Jansen A. DLO-Research Institute for Agrobiology and Soil Fertility (AB-DLO), P.O. Box 14, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 179/1(1996-02-01), 81-87 0032-079X 179:1<81 1996 179 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer