caa a22 4500 477111270 CHVBK 20180405111609.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00009337 doi (NATIONALLICENCE)springer-10.1007/BF00009337 Tn5 insertion mutants of Pseudomonas sp. 267 defective in siderophore production and their effect on clover ( Trifolium pratense ) nodulated with Rhizobium leguminosarum bv. trifolii [Elektronische Daten] [Monika Marek-Kozaczuk, Mieczysawa Deryto, Anna Skorupska] Pseudomonas sp. strain 267 isolated from soil promoted growth of different plants under field conditions and enhanced symbiotic nitrogen fixation in clover under gnotobiotic conditions. This strain produced pyoverdine-like compound under low-iron conditions and secreted vitamins of the B group. The role of fluorescent siderophore production in the beneficial effect of strain 267 on nodulated clover plants was investigated. Several non-fluorescent (Pvd-) Tn5 insertion mutants of Pseudomonas sp. strain 267 were isolated and characterized. The presence of Tn5 insertions was confirmed by Southern analysis of EcoRI digested genomic DNA of each derivative strain. The siderophore-negative mutants were compared to the parental strain with respect to their growth promotion of nodulated clover infected with Rhizobium leguminosarum bv. trifolii 24.1. We found that all isolated Pvd- mutants stimulated growth of nodulated clover plants in a similar manner to the parental strain. No consistent differences were observed between strain 267 and Pvd- derivatives strains with respect to their plant growth promotion activity under gnotobiotic conditions. Kluwer Academic Publishers, 1996 plant growth promotion nationallicence Pseudomonas sp nationallicence Rhizobium leguminosarum bv. trifolii nationallicence siderophore nationallicence Tn5 mutants nationallicence Marek-Kozaczuk Monika Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut Deryto Mieczysawa Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut Skorupska Anna Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut Plant and Soil Kluwer Academic Publishers 179/2(1996-02-01), 269-274 0032-079X 179:2<269 1996 179 11104 https://doi.org/10.1007/BF00009337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00009337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marek-Kozaczuk Monika Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Deryto Mieczysawa Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut NATIONALLICENCE 700 1- Skorupska Anna Department of General Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL 20-033, Lublin, Poland aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers 179/2(1996-02-01), 269-274 0032-079X 179:2<269 1996 179 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer