caa a22 4500 477111343 CHVBK 20180405111609.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF00009336 doi (NATIONALLICENCE)springer-10.1007/BF00009336 Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves [Elektronische Daten] [Bin Yan, Qiujie Dai, Xiaozhong Liu, Shaobai Huang, Zixia Wang] Flooding effects on membrane permeability, lipid peroxidation and activated oxygen metabolism in corn (Zea mays L.) leaves were investigated to determine if activated oxygens are involved in corn flooding-injury. Potted corn plants were flooded at the 4-leaf stage in a controlled environment. A 7-day flooding treatment resulted in a significant increase in chlorophyll breakdown, lipid peroxidation (malondialdehye content), membrane permeability, and the production of superoxide (O 2 - ) and hydrogen peroxide (H2O2) in corn leaves. The effects were much greater in older leaves than in younger ones. Spraying leaves with 8-hydroxyquinoline (an O 2 - scavenger) and sodium benzoate (an .OH scavenger) reduced the oxidative damage and enhanced superoxide dismutase (SOD) activity. A short duration flooding treatment elevated the activities of SOD, catalase, ascorbate peroxidase (AP), and glutathione reductase (GR), while further flooding significantly reduced the enzyme activities but enhanced the concentrations of ascorbic acid and reduced form glutathione (GSH). It was noted that the decline in SOD activity was greater than that in H2O2 scavengers (AP and GR). The results suggested that O 2 - induced lipid peroxidation and membrane damage, and that excessive accumulation of O 2 - is due to the reduced activity of SOD under flooding stress. Kluwer Academic Publishers, 1996 activated oxygen nationallicence activated oxygen scavenging system nationallicence flooding nationallicence lipid peroxidation nationallicence membrane injury nationallicence Zea mays nationallicence AP : ascorbate peroxidase nationallicence ASA : ascorbic acid nationallicence CAT : catalase nationallicence DDTT : sodium diethyldithiocarbamate nationallicence H2O2 : hydrogen peroxide nationallicence 8-HQ-8 : hydroxyquinoline nationallicence GR : glutathione reductase nationallicence GSH : reduced form glutathione nationallicence MDA : malondialdehye nationallicence O2- : superoxide nationallicence .OH : hydroxyl radical nationallicence SB : sodium benzoate nationallicence SOD : superoxide dismutase nationallicence Yan Bin Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut Dai Qiujie Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut Liu Xiaozhong Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut Huang Shaobai Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut Wang Zixia Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut Plant and Soil Kluwer Academic Publishers 179/2(1996-02-01), 261-268 0032-079X 179:2<261 1996 179 11104 https://doi.org/10.1007/BF00009336 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00009336 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Bin Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut NATIONALLICENCE 700 1- Dai Qiujie Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut NATIONALLICENCE 700 1- Liu Xiaozhong Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut NATIONALLICENCE 700 1- Huang Shaobai Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut NATIONALLICENCE 700 1- Wang Zixia Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, Jiangsu, P. R. China aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers 179/2(1996-02-01), 261-268 0032-079X 179:2<261 1996 179 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer