caa a22 4500 477111483 CHVBK 20180405111610.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00015409 doi (NATIONALLICENCE)springer-10.1007/BF00015409 Characterization of rhizobia nodulating Lotus subbiflorus from Uruguayan soils [Elektronische Daten] [P. Irisarri, F. Milnitsky, J. Monza, E. Bedmar] Generation times, acid production, carbon utilization, immunological properties, plasmid content, protein profile and symbiotic properties of 15 isolates of rhizobia nodulating Lotus subbiflorus were studied. Based on specific growth rates, carbon source utilization and acid production, 13 out of the 15 isolates could be assigned to the slow-growing group of rhizobia (bradyrhizobia). Using antisera against whole cells of three isolates, we separated the 15 isolates into three serogroups. Only the slow-growing isolate Ls4 and the fast-growers Ls5 and Ls552 lacked cross-reactivity with any of the sera tested. Electrophoretic mobilities of whole cell protein from seven out of the eight isolates included in the serogroup represented by strain Ls31 were identical. Similarly, isolates Ls1B3 and Ls1B4, both in serogroup Ls1B3, had the same pattern of cell proteins. In contrast, isolates Ls3 and Ls7, belonging to serogroup Ls7, differed in protein profile. Plant growth experiments carried out under bacteriologically controlled conditions revealed that all of the isolates effectively nodulated L. subbiflorus and L. pedunculatus, but were unable to form effective nodules on L. tenuis and L. corniculatus. All isolates showed similar effectiveness in symbiosis with L. subbiflorus, except isolate Ls7, which gave significantly higher plant dry weight. Kluwer Academic Publishers, 1996 Bradyrhizobium nationallicence carbon utilization nationallicence ELISA nationallicence Lotus subbiflorus nationallicence native isolates nationallicence protein profiles nationallicence Rhizobium nationallicence symbiotic effectiveness nationallicence ELISA : enzyme linked immuno-sorbent assay nationallicence kDa : kiloDalton nationallicence MM : mineral medium nationallicence PBS : phosphate-buffered saline nationallicence RE : relative efficiency nationallicence SDS-PAGE : sodium dodecyl sulfate-polyacrylamide gel electrophoresis nationallicence YEM : deyeast extract mannitol nationallicence Irisarri P. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut Milnitsky F. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut Monza J. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut Bedmar E. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zadín, CSIC, PO Box 419, 18080, Granada, Spain aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 180/1(1996-03-01), 39-47 0032-079X 180:1<39 1996 180 11104 https://doi.org/10.1007/BF00015409 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00015409 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Irisarri P. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Milnitsky F. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Monza J. Departamento de Bioquimica, Facultad de Agronomía, Garzón 780, 12900, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Bedmar E. Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zadín, CSIC, PO Box 419, 18080, Granada, Spain aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 180/1(1996-03-01), 39-47 0032-079X 180:1<39 1996 180 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer