caa a22 4500 477111602 CHVBK 20180405111610.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00015422 doi (NATIONALLICENCE)springer-10.1007/BF00015422 Induction of peroxidase by Fe2+ in detached rice leaves [Elektronische Daten] [Xin-xiang Peng, Xi-long Yu, Ming-qi Li, Minoru Yamauchi] Peroxidase (POD) activity was significantly stimulated in detached rice leaves by Fe2+. By electric focusing with thin-layer scanning, three new bands were identified in Fe2+-treated leaves, and the activities of other isozymes were also drastically increased, of which a band with a pI of ca. 9.8 was enhanced from trace amount (invisible) in the control to a large band in the Fe2+-treated leaves. How Fe2+ induced POD activity was investigated. Cycloheximide completely blocked the stimulation of POD activity by Fe2+, but actinomycin D could not inhibit it effectively, indicating that the stimulated activity resulted from inducing de novo biosynthesis of the enzyme at translational level rather than at transcriptional level. When same molar concentration of H2O2 as Fe2+ and sublethal and lethal concentrations of paraquat were introduced to the rice leaves, no significant stimulation of POD activity was observed compared with Fe2+-induced activity, and scavengers of free radicals still could not inhibit the induction of POD by Fe2+. Several chemicals such as EGTA, LaCl3, CPZ, verapamil, which were known to interfere with the action of calcium/calmodulin, were not able to retard POD induction by Fe2+. From these results it appears that the process of Fe2+-induced POD may not be mediated by free radicals or calcium/calmodulin action. Kluwer Academic Publishers, 1996 Fe2+ nationallicence inductive mechanism nationallicence peroxidase nationallicence rice nationallicence EGTA : Ethylene glycol bis (β-aminoethyl ether) nationallicence N,N,N′,N′ : tetra-acetic acid nationallicence DMSO : dimethyl sulfoxide nationallicence CPZ : chlorpromazine nationallicence CH : cycloheximide nationallicence AD : actinomycin D nationallicence POD : peroxidase nationallicence ASC : ascorbate nationallicence GSH : reduced glutathione nationallicence Peng Xin-xiang Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut Yu Xi-long Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut Li Ming-qi Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut Yamauchi Minoru International Rice Research Institute (IRRI), P.O. Box 933, 1099, Manila, Philippines aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 180/1(1996-03-01), 159-163 0032-079X 180:1<159 1996 180 11104 https://doi.org/10.1007/BF00015422 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00015422 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Xin-xiang Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Yu Xi-long Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Li Ming-qi Department of Biology, South China Agricultural University, 510642, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Yamauchi Minoru International Rice Research Institute (IRRI), P.O. Box 933, 1099, Manila, Philippines aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 180/1(1996-03-01), 159-163 0032-079X 180:1<159 1996 180 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer