caa a22 4500 477112005 CHVBK 20180405111611.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00010996 doi (NATIONALLICENCE)springer-10.1007/BF00010996 The infuence of native rhizobacteria on European alder ( Alnus glutinosa (L.) Gaertn.) growth [Elektronische Daten] II. Characterisation and biological assays of metabolites from growth promoting and growth inhibiting bacteria [F. Mañero, N. Acero, J. Lucas, A. Probanza] Metabolite production was investigated in four bacterial strains that promoted (plant growth promoting rhizobacteria -PGPR-, B. licheniformis, isolate B.12 and B. pumilus isolate B.3) or inhibited (deleterious rhizobacteria-DRB-, P. fluorescens bv II, isolates P.9 and P.20) growth of nodulated and non-nodulated Alnus glutinosa seedlings. These strains were isolated and characterized from the rhizosphere of a natural alder population, and their biological effects on plant growth determined on previous studies. Biological assays were performed to confirm the observed effects on aerial length (AL), aerial surface (AS), number of leaves (NL) and total nitrogen (TN). According to the high resolution gas chromatography (HRGC) results, PGPR strains produced auxin-like (IAA-1) compounds at levels of 1.736 and 1.790 mg IAA-1 L-1 culture growth medium; however, they did not produce HCN. These compounds are derived from IAA and not from the Trp originated by peptide degradation in culture media. The promoting effect is evidenced when comparing the effects of IAA and the filtered bacterial growth culture medium to control (increases of 64% in aerial surface, 277% in total N content and 32% in aerial length). The deleterious strains produced HCN (1.6 and 2.4 mg kg-1 detected in growth culture medium) and they did not produce IAA-1 compounds. The bacterial culture's-free of bacteria-inhibiting effects were 7% in aerial surface, 240% in total nitrogen content and 15% in aerial length. The results reported here suggest that the interactions that take place in the alder rhizosphere are in a delicate equilibrium. In view of this, the coexistence of PGPR and DRB strains in this environment is unquestionable, and does affect alder health in field conditions. Kluwer Academic Publishers, 1996 Bacillus nationallicence DRB nationallicence hydrocyanic acid (HCN) nationallicence indolacetic acid (IAA) nationallicence PGPR nationallicence Pseudomonas nationallicence N : nodulated nationallicence NN : non-nodulated, IAA-1-auxin-like nationallicence HRGC : high resolution gas chromatography nationallicence TLC : thin layer chromatography nationallicence DRB : deleterious rhizobacteria nationallicence PGPR : plant growth promoting rhizobacteria nationallicence AL : aerial length nationallicence AS : aerial surface nationallicence NL : number of leaves nationallicence TN : total nitrogen nationallicence Mañero F. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut Acero N. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut Lucas J. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut Probanza A. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl 182/1(1996-05-01), 67-74 0032-079X 182:1<67 1996 182 11104 https://doi.org/10.1007/BF00010996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00010996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mañero F. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut NATIONALLICENCE 700 1- Acero N. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut NATIONALLICENCE 700 1- Lucas J. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut NATIONALLICENCE 700 1- Probanza A. Departamento de Biologia Vegetal, Univ. San Pablo CEU, E-28660 Boadilla del Monte, Madrid, Spain aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; gopher://gopher.wkap.nl/ 182/1(1996-05-01), 67-74 0032-079X 182:1<67 1996 182 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer