caa a22 4500 477112331 CHVBK 20180405111612.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF02185568 doi (NATIONALLICENCE)springer-10.1007/BF02185568 pH mapping in transparent gel using color indicator videodensitometry [Elektronische Daten] [Benoit Jaillard, Laurent Ruiz, Jean-Claude Arvieu] The colored pH indicator method introduced by Weisenseel et al. (1979) is particularly useful for localizing the zones along roots where acidification/alkalinization occurs. It can also be used to assess the direction and intensity of the proton fluxes. Because the method has not been quantitatively evaluated, however, it is nowadays little used or used in conjunction with other such as potentiometry. In the present study we examine the theoretical basis underlying this method of colorimetric visualization and show its similarity to spectrodensitometry. It thus becomes possible to quantify the luminous information and express it in terms of environmental pH. We describe the method used, emphasizing in particular the conditions required to achieve maximum accuracy of measurement, and an appropriate experimental device. pH distribution around roots can be mapped with a relative error of 0.03 pH units. The experimental device is easy to use and incorporates a computer-controlled video camera, thanks to which al acquisition and calculation procedures can be automated. Kluwer Academic Publishers, 1996 agar gel nationallicence color indicator nationallicence pH mapping nationallicence pH rhizosphere nationallicence spectrodensitometry nationallicence video camera nationallicence Jaillard Benoit Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut Ruiz Laurent Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut Arvieu Jean-Claude Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut Plant and Soil Springer Netherlands; gopher://gopher.wkap.nl 183/1(1996-06-01), 85-95 0032-079X 183:1<85 1996 183 11104 https://doi.org/10.1007/BF02185568 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02185568 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jaillard Benoit Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut NATIONALLICENCE 700 1- Ruiz Laurent Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut NATIONALLICENCE 700 1- Arvieu Jean-Claude Laboratoire de Science du Sol, Institut national de la Recherche Agronomique, 2 place Viala, F-34060, Montpellier Cedex 1, France aut NATIONALLICENCE 773 0- Plant and Soil Springer Netherlands; gopher://gopher.wkap.nl/ 183/1(1996-06-01), 85-95 0032-079X 183:1<85 1996 183 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer