caa a22 4500 477112420 CHVBK 20180405111613.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00011443 doi (NATIONALLICENCE)springer-10.1007/BF00011443 Microbiological differences between limed and unlimed soils and their relationship with cavity spot disease of carrots ( Daucus carota L.) caused by Pythium coloratum in Western Australia [Elektronische Daten] [Khaled El-Tarabily, Giles Hardy, Krishnapillai Sivasithamparam, Ipek Kurtböke] Application of lime (4000 kg ha-1) to a soil used for commercial carrot production (pH 6.9) significantly (p<0.05) reduced the incidence of cavity spot disease of carrots compared to unlimed soil (pH 5.1). It significantly (p<0.01) increased soil microbial activity as measured by the hydrolysis of fluorescein diacetate and arginine ammonification. The application of lime resulted in a significant (p<0.01) increase in the total numbers of colony forming units (efu) of aerobic bacteria, fluorescent pseudomonads, Gram negative bacteria, actinomycetes and a significant (p<0.01) decrease in the cfu of filamentous fungi and yeasts compared to unlimed soil. Liming also increased the cfu of non-streptomycete actinomycetes rarely reported in similar studies. These non-streptomycete actinomycetes were estimated and isolated using polyvalent Streptomyces phages and the dry heat technique to reduce the dominance of streptomycetes on isolation plates. The non-streptomycete actinomycetes isolated included species of Actinoplanes, Micromonospora, Streptoverticillium, Nocardia, Rhodococcus, Microbispora, Actinomadura, Dactylosporangium and Streptosporangium. The numbers of actinomycetes antagonistic to Pythium coloratum, a causal agent of cavity spot disease of carrots increased in soil amended with lime. Application of lime also reduced the isolation frequency of P. coloratum from asymptomatic carrot roots grown in soil artificially infested with the pathogen, 3, 4 and 5 weeks after sowing. Kluwer Academic Publishers, 1996 actinomycetes nationallicence antagonism nationallicence cavity spot nationallicence disease suppression nationallicence microbial activity nationallicence Pythium coloratum nationallicence El-Tarabily Khaled School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut Hardy Giles School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut Sivasithamparam Krishnapillai Department of Soil Science and Plant Nutrition, School of Agriculture, The University of Western Australia, 6009, Nedlands, WA, Australia aut Kurtböke Ipek School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 183/2(1996-09-01), 279-290 0032-079X 183:2<279 1996 183 11104 https://doi.org/10.1007/BF00011443 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00011443 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- El-Tarabily Khaled School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut NATIONALLICENCE 700 1- Hardy Giles School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut NATIONALLICENCE 700 1- Sivasithamparam Krishnapillai Department of Soil Science and Plant Nutrition, School of Agriculture, The University of Western Australia, 6009, Nedlands, WA, Australia aut NATIONALLICENCE 700 1- Kurtböke Ipek School of Biological and Environmental Sciences, Murdoch University, 6150, Murdoch, WA, Australia aut NATIONALLICENCE 773 0- Plant and Soil Kluwer Academic Publishers; http://www.wkap.nl 183/2(1996-09-01), 279-290 0032-079X 183:2<279 1996 183 11104 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer