caa a22 4500 477112889 CHVBK 20180405111614.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01746202 doi (NATIONALLICENCE)springer-10.1007/BF01746202 Paracrine effect of human vascular endothelial cells on human vascular smooth muscle cell proliferation: Transmembrane co-culture method [Elektronische Daten] [Hiroaki Yoshida, Motoyuki Nakamura, Shinji Makita, Katsuhiko Hiramori] Summary: To investigate the role endothelial cells have on underlying smooth muscle cell proliferation, human aortic vascular smooth muscle cells were co-cultured with human aortic endothelial cells at different cell densities, using a transmembrane co-culture method. Subconfluent endothelial cells subseeded at low (0.5 × 104 cells/well) and medium densities (2.0 × 104 cells/well) stimulated smooth muscle cell proliferation by 43 ± 14% (P < 0.01) and 39 ± 8% (P < 0.02), respectively. However, this stimulatory effect on smooth muscle cell proliferation was not evident in confluent endothelial cells subseeded at high cell density (8.0 × 104 cells/well). Treatment of smooth muscle cells with trapidil, at 10−6M, for anti-platelet derived growth factor (PDGF) effect or with endothelin-1 receptor blocker FR 139317, at 10−6M failed to inhibit this stimulatory effect. These results imply that subconfluent human endothelial cells are able to exert a stimulatory effect on human smooth muscle cell proliferation, and that this endothelial paracrine growth effect may not be mediated by endothelin or PDGF. Springer-Verlag, 1996 Vascular smooth muscle nationallicence Endothelium nationallicence Endothelin nationallicence PDGF nationallicence Yoshida Hiroaki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Nakamura Motoyuki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Makita Shinji Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Hiramori Katsuhiko Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Heart and Vessels Springer-Verlag 11/5(1996-09-01), 229-233 0910-8327 11:5<229 1996 11 380 https://doi.org/10.1007/BF01746202 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01746202 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshida Hiroaki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Nakamura Motoyuki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Makita Shinji Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Hiramori Katsuhiko Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 773 0- Heart and Vessels Springer-Verlag 11/5(1996-09-01), 229-233 0910-8327 11:5<229 1996 11 380 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer