caa a22 4500 477112900 CHVBK 20180405111614.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF01746201 doi (NATIONALLICENCE)springer-10.1007/BF01746201 Autocrine growth inhibition of IL-1β-treated cultured human aortic smooth muscle cells: Possible role of nitric oxide [Elektronische Daten] [Shinji Makita, Motoyuki Nakamura, Hiroaki Yoshida, Katsuhiko Hiramori] Summary: This study was designed to investigate whether interleukin (IL)-1β would stimulate nitric oxide (NO) production in cultured human aortic vascular smooth muscle cells (VSMCs), and to determine the basic effect of the liberated NO on VSMC proliferation. NO production was estimated from nitrite concentration of culture medium in multi-well plates, determined by the Griess method. VSMCs were IL-1β-pretreated in insert cups, and co-cultured with untreated VSMC in the wells.3H-thymidine (3H-Tdr) incorporation into the VSMC in wells was evaluated for VSMC proliferative activity. IL-1β stimulated NO production in VSMCs in a concentration-dependent manner. This effect was further enhanced by the addition of a membrane-permeable cyclic adenosine monophosphate derivative, dibutyryl cyclic AMP (db-cAMP), and was significantly reduced by concomitant use of an NO synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME). IL-1β-pretreated VSMCs significantly inhibited3H-Tdr incorporation of the co-cultured VSMC. This inhibitory effect was significantly enhanced by the addition of db-cAMP, while this inhibition was significantly decreased by preincubation withl-NAME, and was abolished in thel-arginine-free medium. These results suggest that, in human VSMC, IL-1β stimulates NO production that is enhanced by intracellular cAMP accumulation, and that the liberated NO inhibits further VSMC proliferation in an autocrine fashion. Springer-Verlag, 1996 Vascular smooth muscle cell nationallicence Nitric oxide nationallicence Co-culture nationallicence cAMP nationallicence Nitric oxide synthase inhibitor nationallicence Makita Shinji Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Nakamura Motoyuki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Yoshida Hiroaki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Hiramori Katsuhiko Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut Heart and Vessels Springer-Verlag 11/5(1996-09-01), 223-228 0910-8327 11:5<223 1996 11 380 https://doi.org/10.1007/BF01746201 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01746201 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Makita Shinji Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Nakamura Motoyuki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Yoshida Hiroaki Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Hiramori Katsuhiko Second Department of Internal Medicine, Iwate Medical University, 19-1 Uchimaru, 020, Morioka, Iwate, Japan aut NATIONALLICENCE 773 0- Heart and Vessels Springer-Verlag 11/5(1996-09-01), 223-228 0910-8327 11:5<223 1996 11 380 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer