caa a22 4500 477116906 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01874833 doi (NATIONALLICENCE)springer-10.1007/BF01874833 Characterization of vitellogenin induced by estradiol-17β in protandrous black porgy, Acanthopagrus schlegeli [Elektronische Daten] [Ching-Fong Chang, En-Lieng Lau, Bih-Yun Lin, Shan-Ru Jeng] The objectives of this study were to isolate and characterize vitellogenin from plasma of estradiol-treated protandrous black porgy,Acanthopagrus schlegeli. Vitellogenin concentrations in plasma measured by a validated enzyme-linked immunosorbent assay (ELISA) were also compared. Two-year-old black porgy (n=20) were fed with estradiol-17β (4 mg kg−1 of feed). Plasma was collected for purification of vitellogenin. Two forms of vitellogenin were found in plasma after chromatography on Sepharose CL-2B column and hydroxylapatite, and polyacrylamide gel electrophoresis. Black porgy vitellogenins are phospho-lipo-glycoproteins based on their chemical staining properties. The apparent molecular weights of the two forms of vitellogenin were 636 kDa and 321 kDa, respectively. The amino acid composition of purified vitellogenin was also analyzed after acid hydrolysis. The presence of immunoreactive vitellogenin was identified in the plasma and mucus extract from control and estradiol-induced females on the basis of Western blotting. Serial dilution of the plasma and mucus extract taken from estradiol-induced black porgy showed reactivity to an antiserum against lipovitellin in the ELISA, whereas mucus extract and plasma from male fish did not. Significantly higher concentrations of plasma vitellogenin were detected in estradiol-stimulated black porgy than in the control males. Kugler Publication bv, 1996 Acanthopagrus schlegeli nationallicence black porgy nationallicence estradiol-17β nationallicence mucus nationallicence purification nationallicence sex reversal nationallicence vitellogenin nationallicence Chang Ching-Fong Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut Lau En-Lieng Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut Lin Bih-Yun Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut Jeng Shan-Ru Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 11-19 0920-1742 15:1<11 1996 15 10695 https://doi.org/10.1007/BF01874833 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01874833 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Ching-Fong Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut NATIONALLICENCE 700 1- Lau En-Lieng Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut NATIONALLICENCE 700 1- Lin Bih-Yun Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut NATIONALLICENCE 700 1- Jeng Shan-Ru Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan 20224, Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 11-19 0920-1742 15:1<11 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer