caa a22 4500 477116914 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01874832 doi (NATIONALLICENCE)springer-10.1007/BF01874832 Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout ( Oncorhynchus mykiss ) semen [Elektronische Daten] [Andrzej Ciereszko, Li Liu, Konrad Dabrowski] During the reproductive season, rainbow trout spermatozoa are stored in the sperm ducts for several months. There is no sperm production at this time since spermatogenesis is completed before spawning. To leam more about characteristics of semen during such a long storage, we analyzed changes in protein concentrations, anti-proteinase activity in seminal plasma and sperm aspartate aminotransferase activity during an extended reproductive period during which fish were fed diets supplemented with various ascorbic acid concentrations. Seminal plasma protein concentration and anti-proteinase activity declined toward the end of the reproductive season. These phenomena may be related to oncoming proteolytic events leading to degradation of the sperm. Protein concentrations and anti-proteinase activities were strongly correlated within groups of different ascorbic acid supplementations and several sampling dates (r=0.6-0.9 in most cases, p<0.05). Ascorbic acid deficiency resulted in a decrease in both parameter levels as compared to levels in groups with vitamin C supplement (p<0.08). Deficiency also resulted in lower stimulation of aspartate aminotransferase by an exogenous pyridoxal 5′-phosphate in comparison to fish fed vitamin C-supplemented diets (p<0.05). These results support earlier studies suggesting a protective role of ascorbic acid toward maintaining sperm quality. Kugler Publication bv, 1996 fish nationallicence vitamin C nationallicence spermatozoa nationallicence seminal plasma nationallicence proteinase inhibitors nationallicence aspartate aminotransferase nationallicence Ciereszko Andrzej School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut Liu Li School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut Dabrowski Konrad School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 1-10 0920-1742 15:1<1 1996 15 10695 https://doi.org/10.1007/BF01874832 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01874832 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ciereszko Andrzej School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Liu Li School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Dabrowski Konrad School of Natural Resources, The Ohio State University, 210 Kottman Hall, 2021 Coffey Rd, 43210, Columbus, OH, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 1-10 0920-1742 15:1<1 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer