caa a22 4500 477116922 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01874836 doi (NATIONALLICENCE)springer-10.1007/BF01874836 Tyrosine hydroxylase activity and dopamine turnover of rainbow trout ( Oncorhynchus mykiss ) brain: the special status of the hypothalamus [Elektronische Daten] [Boris Linard, Sanae Bennani, Patrick Jego, Christian Saligaut] The dynamics of catecholamine (CA)-synthesis enzymes have been poorly studied in fish. Tyrosine hydroxylase (TH), the rate-limiting enzyme of CA synthesis has been only studied inin vitro conditions. In the present report thein vivo CA synthesis and the CA metabolism were studied in different regions of the forebrain of the rainbow trout. Levels of norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and the rate of accumulation of 3,4-dihydroxyphenylalanine (DOPA) were determined by HPLC following a treatment with hydroxybenzylhydrazine (NSD), a potential inhibitor of DOPA decarboxylase. Kinetics of the accumulation of DOPA and of the decline of DOPAC were in agreement with those found in rat, evidencing that the accumulation of DOPA following NSD can be used in trout to quantify thein vivo enzymatic activity of tyrosine hydroxylase. Experiments using treatment with NSD or with methyl-p-tyrosine reached a same conclusion: the DA neuronal activity in trout is much higher than NE neuronal activity. However, the hypothalamus had high DA levelsvs. lowin vitro andin vivo TH activities and exhibited a low CA turnover. Kugler Publication bv, 1996 trout nationallicence dopamine nationallicence norepinephrine nationallicence tyrosine hydroxylase nationallicence olfactory bulbs nationallicence hypothalamus nationallicence preoptic area nationallicence telencephalon nationallicence Linard Boris Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut Bennani Sanae Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut Jego Patrick Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut Saligaut Christian Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 41-48 0920-1742 15:1<41 1996 15 10695 https://doi.org/10.1007/BF01874836 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01874836 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linard Boris Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut NATIONALLICENCE 700 1- Bennani Sanae Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut NATIONALLICENCE 700 1- Jego Patrick Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut NATIONALLICENCE 700 1- Saligaut Christian Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes I, Campus de Beaulieu, 35042, Rennes cedex, France aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 41-48 0920-1742 15:1<41 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer