caa a22 4500 477116930 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01874834 doi (NATIONALLICENCE)springer-10.1007/BF01874834 Wiegand Murray Department of Biology, University of Winnipeg, 515 Portage Avenue, R3B 2E9, Winnipeg, Manitoba, Canada aut Utilization of yolk fatty acids by goldfish embryos and larvae [Elektronische Daten] [Murray Wiegand] In each of two separate experiments, eggs from a single female goldfish were fertilized, incubated at 22°C and sampled regularly up to day 6 when the larvae could be expected to commence feeding. Hatching normally occurred on Day 4. Lipids were extracted from the eggs and larvae and the neutral lipid and neutral phospholipids were isolated on aminopropyl columns. Fatty acid analysis of the eggs revealed the typical situation in fish where the phospholipids were rich in polyunsaturated fatty acids (PUFA) and the neutral lipids were rich in monounsaturated fatty acids (MUFA). Assay of lipid masses revealed that little depletion of lipid occurred until after hatch and that the neutral phospholipids were the principal fraction consumed. Although the neutral lipid mass did not change substantially during development, its fatty acid profile did. The proportions of several PUFA in the neutral lipids, especially 22∶6(n−3), 20∶5(n−3) and 20∶4(n−6), increased substantially during development while proportions of MUFA and 18∶2(n−6) declined. This appears to be a mechanism by which the larva can retain essential fatty acid released on hydrolysis of phospholipid while deriving the benefits of catabolism of phospholipid as fuel, namely the provision of phosphate and choline for intermediary metabolism and for the synthesis of macromolecules and neurotransmitter. Kugler Publication bv, 1996 goldfish nationallicence eggs nationallicence embryos nationallicence larvae nationallicence lipids nationallicence fatty acids nationallicence neutral lipids nationallicence phospholipids nationallicence AA : arachidonic acid (20∶4(n−6)) nationallicence DHA : docosahexaenoic acid (22∶6(n−3)) nationallicence EPA : eicosapentaenoic acid (20∶5(n−3)) nationallicence MUFA : monounsaturated fatty acid nationallicence PC : phosphatidylcholine nationallicence PE : phosphatidylethanolamine nationallicence PUFA : polyunsaturated fatty acid nationallicence SFA : saturated fatty acid nationallicence Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 21-27 0920-1742 15:1<21 1996 15 10695 https://doi.org/10.1007/BF01874834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01874834 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Wiegand Murray Department of Biology, University of Winnipeg, 515 Portage Avenue, R3B 2E9, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 21-27 0920-1742 15:1<21 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer