caa a22 4500 477116949 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01874840 doi (NATIONALLICENCE)springer-10.1007/BF01874840 Qualitative and quantitative strategies of thermal adaptation of grass carp ( Ctenopharyngodon idella ) cytoplasmic malate dehydrogenases [Elektronische Daten] [Jen-jen Lin, Shirley MacLeod, Ching-ming Kuo] Malate dehydrogenase isozymes of grass carp,Ctenopharyngodon idella, were identified by mitochondrial preparation and thermal denaturation. The structural and kinetic characteristics of chromatographically separated thermostable and thermolabile cMDHs were different in (1) half-life at 42°C, 10 min and 24 h, respectively, (2) optimal substrate, oxaloacetate and malate, concentrations, and (3) the apparent Michaelis-Menten constants of NADH and oxaloacetate. Total MDH activity in white muscle of 11°C-acclimated fish was about twice that of the 30°C-acclimated group. In addition, the ratio of the thermostable to thermolabile cMDH activity in white muscle of 30°C-acclimated fish was significantly higher than that of 11°C-acclimated fish. These results suggest that temperature acclimation can induce temperature compensation in MDH activity and differential expression of thermostable and thermolabile cMDH isozymes in freshwater fish. Kugler Publication bv, 1996 temperature nationallicence malate dehydrogenase nationallicence acclimation nationallicence adaptation nationallicence grass carp nationallicence Ctenopharyngodon idella nationallicence teleost fish nationallicence thermostability nationallicence isozyme nationallicence kinetics nationallicence Lin Jen-jen Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut MacLeod Shirley Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut Kuo Ching-ming Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 71-81 0920-1742 15:1<71 1996 15 10695 https://doi.org/10.1007/BF01874840 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01874840 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Jen-jen Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut NATIONALLICENCE 700 1- MacLeod Shirley Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut NATIONALLICENCE 700 1- Kuo Ching-ming Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/1(1996-02-01), 71-81 0920-1742 15:1<71 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer