caa a22 4500 477117074 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01875577 doi (NATIONALLICENCE)springer-10.1007/BF01875577 Response of rainbow trout gill (Na++K+)-ATPase and chloride cells to T3 and NaCl administration [Elektronische Daten] [Fabiana Trombetti, Vittoria Ventrella, Alessandra Pagliarani, Rodolfo Ballestrazzi, Marco Galeotti, Gianni Trigari, Maurizio Pirini, Anna Borgatti] With the aim of comparing the effects of oral T3 and NaCl administration on trout hypoosmoregulatory mechanisms, three groups of rainbow trout (Oncorhynchus mykiss Walbaum) held in freshwater (FW) were fed a basal diet (C), the same diet containing 8.83 ppm of 3,5,3′-triiodo-L-thyronine (T3) (T) or 10% (w/w) NaCl (N) respectively for 30 d. They were then transferred to brackish water (BW) for 22 d and fed on diet C. Gill (Na++K+)-ATPase activity and its dependence on ATP, Na+ and pH, number of gill chloride cells (CC), serum T3 level as well as fish growth, condition factor (K) and mortality were evaluated. During the FW phase, as compared to C trout, T trout showed a two fold higher serum T3 level, had unchanged gill (Na++K+)-ATPase activity and increased CC number, whereas N trout showed higher gill (Na++K+)-ATPase activity and CC number. At the end of the experiment the enzyme activity was in the order T>N>C groups and all groups showed similar CC number. Both treatments changed the enzyme activation kinetics by ATP and Na+. A transient increase in K value occurred in N group during the period of salt administration. In BW, T and N groups had higher and lower survival than C group respectively. Other parameters were unaffected by the treatments. This trial suggests that T3 administration promotes the development of hypoosmoregulatory mechanisms of trout but it leaves the (Na++K+)-ATPase activity unaltered till the transfer to a hyperosmotic environment. Kugler Publication, 1996 rainbow trout nationallicence T3 nationallicence salt diet nationallicence gill (Na++K+)-ATPase nationallicence chloride cells nationallicence salinity adaptation nationallicence Trombetti Fabiana Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Ventrella Vittoria Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Pagliarani Alessandra Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Ballestrazzi Rodolfo Dipartimento di Scienze della Produzione Animale, University of Udine, Italy aut Galeotti Marco Dipartimento di Scienze della Produzione Animale, University of Udine, Italy aut Trigari Gianni Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Pirini Maurizio Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Borgatti Anna Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/3(1996-06-01), 265-274 0920-1742 15:3<265 1996 15 10695 https://doi.org/10.1007/BF01875577 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01875577 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Trombetti Fabiana Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 700 1- Ventrella Vittoria Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 700 1- Pagliarani Alessandra Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 700 1- Ballestrazzi Rodolfo Dipartimento di Scienze della Produzione Animale, University of Udine, Italy aut NATIONALLICENCE 700 1- Galeotti Marco Dipartimento di Scienze della Produzione Animale, University of Udine, Italy aut NATIONALLICENCE 700 1- Trigari Gianni Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 700 1- Pirini Maurizio Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 700 1- Borgatti Anna Dipartimento di Biochimica "G. Moruzzi”, University of Bologna, Italy aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/3(1996-06-01), 265-274 0920-1742 15:3<265 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer