caa a22 4500 477117104 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960601xx s 000 0 eng 10.1007/BF01875576 doi (NATIONALLICENCE)springer-10.1007/BF01875576 Identification and characterization of maturation-promoting factor from catfish, Clarias batrachus [Elektronische Daten] [S. Haider, K. Balamurugan] Maturation-promoting factor (MPF) extracted from maturing oocytes of catfishes (Clarias batrachus andHeteropneustes fossilis) and carp (Labeo rohita) induces 100% germinal vesicle breakdown (GVBD) when microinjected intoClarias immature unstimulated oocytes. The presence of a similar MPF activity has also been demonstrated in the active fractions collected after superose 12. SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34cdc2 recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [35S]methionine was observed mainly in the region of 46 kDa protein band indicatingde novo synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. These findings indicate the presence of p34cdc2 homologs and cyclin B in the MPF of the catfishes and carp oocytes. Kugler Publication, 1996 catfish nationallicence maturation-promoting factor nationallicence identification nationallicence characterization nationallicence Haider S. Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India aut Balamurugan K. Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/3(1996-06-01), 255-263 0920-1742 15:3<255 1996 15 10695 https://doi.org/10.1007/BF01875576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01875576 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haider S. Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India aut NATIONALLICENCE 700 1- Balamurugan K. Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/3(1996-06-01), 255-263 0920-1742 15:3<255 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer