caa a22 4500 477117171 CHVBK 20180405111625.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02112360 doi (NATIONALLICENCE)springer-10.1007/BF02112360 Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos [Elektronische Daten] [Kozo Inaba, Masaharu Seno, Hisaya Morino] Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4-8 and 12-16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12-16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4-8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4-8 somite embryos. In the heparin-binding fraction derived from 12-16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable. Kugler Publication bv Amsterdam, 1996 fish FGF-1 nationallicence fish FGF-2 nationallicence fish embryos nationallicence cell proliferation nationallicence heparin affinity nationallicence Western blot nationallicence Inaba Kozo Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14, Hyogo, Japan aut Seno Masaharu Department of Bioengineering Science, Faculty of Engineering, Okayama University, 700, Tsushima, Okayama, Japan aut Morino Hisaya Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14, Hyogo, Japan aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 333-338 0920-1742 15:4<333 1996 15 10695 https://doi.org/10.1007/BF02112360 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02112360 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inaba Kozo Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14, Hyogo, Japan aut NATIONALLICENCE 700 1- Seno Masaharu Department of Bioengineering Science, Faculty of Engineering, Okayama University, 700, Tsushima, Okayama, Japan aut NATIONALLICENCE 700 1- Morino Hisaya Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14, Hyogo, Japan aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 333-338 0920-1742 15:4<333 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer