caa a22 4500 477117201 CHVBK 20180405111626.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02112356 doi (NATIONALLICENCE)springer-10.1007/BF02112356 Effects of Zn2+ on Ca2+ uptake by mitochondria and endoplasmic reticulum in permeabilized tilapia gill cells [Elektronische Daten] [P. Verbost, M. Salah El-Deen, P. Pelt, M. Bijvelds, S. Wendelaar Bonga] An intracellular ATP-dependent Ca2+ pumping mechanism, distinct from mitochondrial Ca2+ accumulation, was identified within tilapia gill cells. Cell suspensions treated with 0.003% saponin, which selectively permeabilizes the plasma membrane, were used to characterize the Ca2+ sequentering mechanisms as endoplasmic reticulum and mitochondria and to determine the effect of Zn2+ on their Ca2+ storing activity. Of the Ca2+ taken up by the endoplasmic reticulum, 80% was released by IP3 (10 μmol l−1). The Ca2+ pump of the endoplasmic reticulum was 2.5 times less sensitive to Zn2+ (IC50=0.05 nmol l−1) than was the mitochondrial uptake mechanism (IC50=0.20 nmol l−1). The results indicate that Ca2+ is stored predominantly within the endoplasmic reticulum at 0.1 μmol l−1 and that this storing capacity is seriously attenuated by namomolar concentrations Zn2+. Kugler Publication bv Amsterdam, 1996 calcium stores nationallicence calcium accumulation nationallicence gill cells nationallicence zinc nationallicence BSA : bovine serum albumin nationallicence EGTA : ethylene glycol bis-(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid nationallicence HEEDTA : N-(2-hydroxyethyl) ethylene diamine-N,N′,N′-triacetic acid nationallicence HEPES : 4-(2-hydroxyethyl)-1-piperazine ethane sulphonic acid nationallicence NTA : nitrilotriacetic acid nationallicence Verbost P. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut Salah El-Deen M. Water Research Center, Research Institute of Weed Control and Channel Maintenance, Kanater, El Khaireia, Kalubeia, Egypt aut Pelt P. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut Bijvelds M. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut Wendelaar Bonga S. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 299-305 0920-1742 15:4<299 1996 15 10695 https://doi.org/10.1007/BF02112356 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02112356 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verbost P. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Salah El-Deen M. Water Research Center, Research Institute of Weed Control and Channel Maintenance, Kanater, El Khaireia, Kalubeia, Egypt aut NATIONALLICENCE 700 1- Pelt P. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Bijvelds M. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Wendelaar Bonga S. Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525-ED, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 299-305 0920-1742 15:4<299 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer