caa a22 4500 477117236 CHVBK 20180405111626.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02112357 doi (NATIONALLICENCE)springer-10.1007/BF02112357 Tsai Jong-Chang Department of Zoology, National Taiwan University, Taipei, Taiwan R.O.C. aut Characterization of the pattern of cytokeratin proteins in the epidermal cells of loach, Misgurnus anguillicaudatus (Cyprinformes) [Elektronische Daten] [Jong-Chang Tsai] The pattern of cytokeratin proteins in the epidermal cells of loach was studied by immunotechniques and partial separation of the epidermal cells. Two monoclonal antibodies, namely 8F7 and 1C45, against the cytokeratin proteins of the loach epidermis were prepared. these two monoclonal antibodies exhibit distinctive results in immunohistochemical staining. The 8F7 monoclonal antibody stains mainly with the epithelial cells, while the 1C45 monoclonal antibody stains specifically with the club cells. The pattern of cytokeratin proteins in the club cells and the epithelial cells of various epidermal layers was further determined by partial separation of these cells. Immunoblotting analysis of the cell fractions confirms the cytokeratin proteins to be differentially expressed in the club cells and the epithelial cells. However, the cytokeratin proteins expressed in the epithelial cells of the basal, middle and outer layers are same. The results indicate that differentiation of the epithelial cells seems limited during their translocation from basal to upper layers, but in those cells that do differentiate into club cells, the cytokeratin pattern changes. Kugler Publication bv Amsterdam, 1996 cytokeratin nationallicence epidermis nationallicence loach nationallicence monoclonal antibody nationallicence immunohistochemistry nationallicence immunoblotting nationallicence cell separation nationallicence cell differentiation nationallicence Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 307-316 0920-1742 15:4<307 1996 15 10695 https://doi.org/10.1007/BF02112357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02112357 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Tsai Jong-Chang Department of Zoology, National Taiwan University, Taipei, Taiwan R.O.C aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 307-316 0920-1742 15:4<307 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer