caa a22 4500 477117244 CHVBK 20180405111626.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF02112354 doi (NATIONALLICENCE)springer-10.1007/BF02112354 Basal H2-receptor stimulated and pH-dependent acid secretion from an isolated stomach mucosa preparation of the cod, Gadus morhua , studied using a modified pH-static titration method [Elektronische Daten] [Peter Bomgren, Ann-Cathrine Jönsson] Gastric acid secretion from isolated cod stomach mucosa was measured using a pH-static titration method. A basal acid secretion rate (BASR) of 6.0±0.6 nEqH+min−1cm−1 was measured when using 0.9% NaCl as luminal solution. There was a dose-dependent increase in response to histamine between 0.12 and 0.20 μM (EC50=0.15 μM), above which gastric acid secretion plateaued at 13.5±1.8 nEqH+min−1cm−1. Ranitidine, a H2-receptor antagonist, completely blocked the stimulatory effect of histamine and reduced the BASR. The H1-receptor antagonist, clemastine, did not inhibit the response to histamine. Acid secretion rates decreased significantly when the pH of the luminal side of the mucosa was lowered from pH 5.75 to pH 4.50, indicating that a negative feedback mechanism was operating. Histological staining showed that oxynticopeptic cells were uniformly distributed throughout the cardiac stomach. It is concluded that the acid secretion in the isolated stomach mucosa of cod can be measuredin vitro with a pH-static titration method. The method was used to demonstrate that the BASR is downregulated by a decrease in pH. Furthermore, we conclude that the histamine receptor in the cod stomach mucosa resembles the mammalian H2-receptor and that histamine is secreted under basal conditions. Kugler Publication bv Amsterdam, 1996 basal acid secretion nationallicence histamine nationallicence ranitidine nationallicence clemastine nationallicence H2-receptor nationallicence pH titration nationallicence oxynticopeptic cells nationallicence teleost nationallicence cod nationallicence Bomgren Peter Department of Zoophysiology, University of Göteborg, Medicinaregatan 18, 413 90, Göteborg, Sweden aut Jönsson Ann-Cathrine Department of Zoophysiology, University of Göteborg, Medicinaregatan 18, 413 90, Göteborg, Sweden aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 275-285 0920-1742 15:4<275 1996 15 10695 https://doi.org/10.1007/BF02112354 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02112354 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bomgren Peter Department of Zoophysiology, University of Göteborg, Medicinaregatan 18, 413 90, Göteborg, Sweden aut NATIONALLICENCE 700 1- Jönsson Ann-Cathrine Department of Zoophysiology, University of Göteborg, Medicinaregatan 18, 413 90, Göteborg, Sweden aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/4(1996-08-01), 275-285 0920-1742 15:4<275 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer