caa a22 4500 47711735X CHVBK 20180405111626.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF01875585 doi (NATIONALLICENCE)springer-10.1007/BF01875585 Characterization of the microsomal epoxide hydrolase of hepatic microsomes of the common dab, Limanda limanda [Elektronische Daten] [Anne McCord, Nicola Dunlop, Ronald Stagg, John Craft] Epoxide hydrolase of microsomal membranes of the common dab (Limanda limanda) has been characterized using p-nitrostyrene oxide as substrate. Under the conditions of assay used, the turnover number with this substrate was higher than found for the more frequently used styrene oxide and steady state kinetics were observed. The enzyme had a KM of 0.12 mM and optima for pH and temperature between pH 8-10.2 and 50-60°C respectively. Enzyme activity was unaffected by low concentrations of ionic and non-ionic detergents but was inhibited by higher concentrations of Lubrol and Brij. The enzyme protein did not react with monospecific antibodies to rat or human microsomal epoxide hydrolase during Western blotting. Large inter-individual variation in enzyme activity was found but the enzyme does not appear to be expressed in a gender-specific way. Fish were administered a wide range of hydrocarbons which are known to alter the expression of cytochrome P450 1A but these had no effect other than benzothiophene which caused a small increase in enzyme activity. Kugler Publication, 1996 microsomal epoxide hydrolase nationallicence mEH nationallicence polyaromatic hydrocarbons nationallicence mEH : microsomal epoxide hydrolase nationallicence pNSG : p-nitrostyrene glycol nationallicence pNSO : p-nitrostyrene oxide nationallicence McCord Anne Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut Dunlop Nicola Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut Stagg Ronald SOAEFD Marine Laboratory, Victoria Road, PO Box 101, AB11 9DB, Aberdeen, Scotland aut Craft John Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/5(1996-11-01), 421-430 0920-1742 15:5<421 1996 15 10695 https://doi.org/10.1007/BF01875585 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01875585 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCord Anne Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut NATIONALLICENCE 700 1- Dunlop Nicola Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut NATIONALLICENCE 700 1- Stagg Ronald SOAEFD Marine Laboratory, Victoria Road, PO Box 101, AB11 9DB, Aberdeen, Scotland aut NATIONALLICENCE 700 1- Craft John Department of Biological Sciences, Glasgow Caledonian University, Cowcaddens Road, G4 OBA, Glasgow aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/5(1996-11-01), 421-430 0920-1742 15:5<421 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer