caa a22 4500 477117376 CHVBK 20180405111626.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01875595 doi (NATIONALLICENCE)springer-10.1007/BF01875595 Characterization of growth hormone binding sites in the goldfish, Carassius auratus [Elektronische Daten] effects of hypophysectomy and hormone injection [Yushi Zhang, Tracy Marchant] A recombinant carp growth hormone (rcGH) was used to develop for a GH radioreceptor binding assay in the goldfish (Carassius auratus). Specific binding of125I-rcGH to goldfish liver membranes was a pH, time, temperature, and membrane protein dependent process. Scatchard and LIGAND analysis indicated a single class of high affinity and low capacity binding site, with an association constant (Ka) of 1.9×1010 M−1 and a maximum binding capacity (Bmax) of 9 fmol mg−1 protein. Liver tissue displayed the highest125I-rcGH binding of all the tissues examined. Displacement of125I-rcGH with various unlabeled teleost and mammalian GHs and prolactins revealed that the goldfish hepatic binding site was highly specific for teleost GH. Intraperitoneal administration of 0.1, 1.0, and 10 μg rcGH g−1 body weight to hypophysectomized goldfish resulted in a 27, 52, and 68% decrease in total binding sites, respectively. Injection of a high dose of rat prolactin (rPRL) (5 μg rPRL g−1 body weight) also resulted in a 32% decrease in total binding sites. These results suggest that endogenous GH may have a role in the regulation of its own receptors in the goldfish. Kugler Publication bv Amsterdam, 1996 growth hormone nationallicence receptor nationallicence hypophysectomy nationallicence liver nationallicence goldfish nationallicence teleost nationallicence Zhang Yushi Department of Biology, University of Saskatchewan, S7N 5E2, Saskatoon, Saskatchewan, Canada aut Marchant Tracy Department of Biology, University of Saskatchewan, S7N 5E2, Saskatoon, Saskatchewan, Canada aut Fish Physiology and Biochemistry Kluwer Academic Publishers 15/2(1996-04-01), 157-165 0920-1742 15:2<157 1996 15 10695 https://doi.org/10.1007/BF01875595 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01875595 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yushi Department of Biology, University of Saskatchewan, S7N 5E2, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Marchant Tracy Department of Biology, University of Saskatchewan, S7N 5E2, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Kluwer Academic Publishers 15/2(1996-04-01), 157-165 0920-1742 15:2<157 1996 15 10695 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer