caa a22 4500 477118488 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00239314 doi (NATIONALLICENCE)springer-10.1007/BF00239314 Effect of acute exercise on glutathione deficient heart [Elektronische Daten] [Christiaan Leeuwenburgh, Steve Leichtweis, John Hollander, Russ Fiebig, Mitch Gore, Li Ji] The role of glutathione (GSH) in myocardial antioxidant defense was investigated in Swiss-Webster mice either performing swim exercise to exhaustion or rested in both the GSH adequate (GSH-A) and GSH deficient (GSH-D) states. GSH deficiency was accomplished by injecting mice with L-buthionine [S,R]sulfoximine (BSO; 2 nmol/kg body wt, i.p.) and providing BSO (20 mM) in drinking water for 12 days. GSH and glutathione disulfide (GSSG) contents in the GSH-D hearts were decreased to 10 and 8%, respectively, of those in the GSH-A mice. This decrease was associated with a significant decline of the total glutathione level in the liver, skeletal muscle and plasma. Myocardial GSH peroxidase and GSH sulfur-transferase activities decreased significantly following GSH deficiency, whereas superoxide dismutase activity was significantly elevated. GSH deficiency did not affect exercise endurance performance. However, exhaustive exercise decreased GSH content in the myocardium of the GSH-A and GSH-D mice by 22 and 44% (p < 0.05), respectively. The GSH:GSSG ratio was not altered significantly following exercise because of a concomitant decrease in GSSG (p < 0.05). γ-Glutamyltranspeptidase activity was significantly increased after exercise, especially in the GSH-D hearts (72%; p < 0.05). GSH content after exercise correlated negatively with exercise time in both GSH-A and GSH-D mice (p < 0.05). These data indicate that GSH is actively used in the myocardium during prolonged exercise at moderate intensity and that GSH deficiency is tolerated by the heart, possibly compensated for by an increased GSH uptake from the plasma. Kluwer Academic Publishers, 1996 antioxidant enzymes nationallicence heart nationallicence buthionine sulfoximine nationallicence GSH-deficiency nationallicence Leeuwenburgh Christiaan Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Leichtweis Steve Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Hollander John Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Fiebig Russ Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Gore Mitch Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Ji Li Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 17-24 0300-8177 156:1<17 1996 156 11010 https://doi.org/10.1007/BF00239314 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00239314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leeuwenburgh Christiaan Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Leichtweis Steve Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Hollander John Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Fiebig Russ Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Gore Mitch Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Ji Li Department of Kinesiology, University of Wisconsin, 53706, Madison, WI, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 17-24 0300-8177 156:1<17 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer