caa a22 4500 477118496 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00239319 doi (NATIONALLICENCE)springer-10.1007/BF00239319 Chronic muscle stimulation increases lactate transport in rat skeletal muscle [Elektronische Daten] [Karl McCullagh, Carsten Juel, Moira O'Brien, Arend Bonen] The aim of this study was to examine the effects of chronic low frequency stimulation on the lactate transport across the plasma membrane of the tibialis anterior (TA) muscle of the rat. Stimulating electrodes were implanted on either side of the peroneal nerve in one hindlimb. Chronic stimulation (10 Hz, 50 psec bursts, 24 h/day) commenced 7 days after surgery, and were continued for 7 days. Animals were then left for 24 h, and thereafter muscles were obtained. Cytochrome C-oxidase activity was increased 1.9-fold in the stimulated TA compared to the control TA (p < 0.05). Lactate transport (zero-trans) was measured in giant sarcolemmal vesicles obtained from the chronically stimulated TA and the control TA. At each of the concentrations used in these studies a significant increase in lactate transport was observed: 2.8-fold increase at 1 mM lactate p < 0.05); 2-fold increases at both 30 mM and 50 mM lactate p < 0.05). These studies have shown that lactate transport capacity is markedly increased in response to chronic muscle contraction. Kluwer Academic Publishers, 1996 electrical stimulation nationallicence giant sarcolemmal vesicles nationallicence muscle activity nationallicence training nationallicence McCullagh Karl Department of Anatomy and Physiology, Trinity College, Dublin, Ireland aut Juel Carsten The Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark aut O'Brien Moira Department of Anatomy and Physiology, Trinity College, Dublin, Ireland aut Bonen Arend Department of Kinesiology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 51-57 0300-8177 156:1<51 1996 156 11010 https://doi.org/10.1007/BF00239319 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00239319 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCullagh Karl Department of Anatomy and Physiology, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Juel Carsten The Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark aut NATIONALLICENCE 700 1- O'Brien Moira Department of Anatomy and Physiology, Trinity College, Dublin, Ireland aut NATIONALLICENCE 700 1- Bonen Arend Department of Kinesiology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 51-57 0300-8177 156:1<51 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer