caa a22 4500 477118518 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00239321 doi (NATIONALLICENCE)springer-10.1007/BF00239321 Induction of early growth response-1 gene by interleukin-1β and tumor necrosis factor-α in normal human bone marrow stromal and osteoblastic cells: regulation by a protein kinase C inhibitor [Elektronische Daten] [Lala Chaudhary, Su-Li Cheng, Louis Avioli] The early growth response-1 (Egr-1) gene has been identified as a nuclear transcriptional factor and implicated in the regulation of growth and differentiation of osteoblastic cells. In the present study, we investigated whether Egr-1 mRNA is expressed and induced by interleukin-1 β (IL-β) and tumor necrosis factor-α (TNF-α) in normal human bond marrow stromal (HBMS) and osteoblastic (HOB) cells. Results demonstrate a very low basal expression of Egr-1 mRNA which is induced by IL-1 β and TNF-α in a time- and dose-dependent manner. Egr-1 mRNA induction was detectable within 15 min, reached maximal by 60 min and thereafter declined to basal levels by 120 min. Induction of Egr-1 mRNA by IL-1β and TNF-α was completely inhibited by H-7 suggesting the mediation of protein kinase C. The induction by IL-1 β and TNF-α of Egr-1 mRNA was independent of de novo protein synthesis since this induction was also observed in the presence of protein synthesis inhibitor cycloheximide. Fetal bovine serum and cycloheximide also independently induced the Egr-1 mRNA. Actinomycin D experiments demonstrated that Egr-1 mRNA is degraded very rapidly with a half-life of 30 min. Our results demonstrate the expression of Egr-1 gene and its induction by IL-1β, and TNF-α in normal human bone marrow stromal (osteoprogenitor) and osteoblastic cells in primary cultures. Data also reveal that the expression of Egr-1 gene is inhibited by protein kinase C inhibitor H-7 suggesting that the activation of protein kinase C or other protein kinases resulting in the phosphorylation of specific transcription factor(s) is the first immediate early step in the induction of immediate-early Egr-1 gene by IL-1 β and TNF-α. Results also suggest that Egr-1 is an important mediator of IL-1 β and TNF-α action in normal human osteoblastic cells. Kluwer Academic Publishers, 1996 human bone marrow stromal cells nationallicence osteoblasts nationallicence Egr-1 nationallicence immediate-early gene nationallicence interleukin-1 β tumor necrosis factor-α nationallicence Chaudhary Lala Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut Cheng Su-Li Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut Avioli Louis Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 69-77 0300-8177 156:1<69 1996 156 11010 https://doi.org/10.1007/BF00239321 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00239321 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaudhary Lala Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Cheng Su-Li Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Avioli Louis Department of Internal Medicine, Division of Bone and Mineral Diseases, Washington University School of Medicine, Jewish Hospital, 63110, St. Louis, MO, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 69-77 0300-8177 156:1<69 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer