caa a22 4500 477118534 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00239320 doi (NATIONALLICENCE)springer-10.1007/BF00239320 Bhullar Rajinder Departments of Oral Biology and, Biochemistry and Molecular Biology, University of Manitoba, R3E OW3, Winnipeg, Manitoba, Canada aut Expression of a 24 kDa GTP-binding protein (Gn24) is increased in lovastatin treated human erythroleukemia cells [Elektronische Daten] [Rajinder Bhullar] A major 27 kDa particulate and a minor 24 kDa cytosolic GTP-binding protein was detected in HEL cells upon incubation with [α-32P]GTP of nitrocellulose blots containing polypeptides separated using SDS-PAGE. Addition of lovastatin (30 μM) to HEL cells in culture inhibited protein synthesis by ∼35%. However, this treatment resulted in a 5-fold increase, as quantitated by [α-32P]GTP binding, in the amount of cytosolic 24 kDa GTP-binding protein. Addition of cycloheximide plus lovastatin to cells in culture abolished the observed increase in 24 kDa GTP-binding protein. Incubation of cells with lovastatin plus [R,S]-[5-3H]mevalonolactone resulted in the incorporation of radioactivity into several polypeptides in both the cytosolic and particulate fractions including a polypeptide of molecular mass of 24 kDa in the cytosol. The mobility of this 24 kDa isoprenylated protein on SDS-PAGE was identical to that of the GTP-binding protein increased in response to lovastatin. However, the 24 kDa protein remained in the cytosol after undergoing isoprenylation. The 24 kDa protein was distinct from the HEL cell, G25K/CDC42Hs GTP-binding protein and the GTP-binding protein that was a substrate for botulinum toxin C3 catalyzed ADP-ribosylation. Results demonstrate that lovastatin specifically increases the expression of a 24 kDa GTP-binding protein in HEL cells and that, isoprenylation of low molecular mass GTP-binding protein(s) may have function(s) in addition to its role in the targetting of these proteins to cell membrane. Kluwer Academic Publishers, 1996 Gn-proteins nationallicence ras -related nationallicence GTP-binding proteins nationallicence lovastatin nationallicence isoprenylation nationallicence human erythroleukemia cell nationallicence Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 59-67 0300-8177 156:1<59 1996 156 11010 https://doi.org/10.1007/BF00239320 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00239320 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bhullar Rajinder Departments of Oral Biology and, Biochemistry and Molecular Biology, University of Manitoba, R3E OW3, Winnipeg, Manitoba, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 59-67 0300-8177 156:1<59 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer