caa a22 4500 477118550 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00239318 doi (NATIONALLICENCE)springer-10.1007/BF00239318 Regulation of c-fos mRNA expression in Sertoli cells by cyclic AMP, calcium, and protein kinase C mediated pathways [Elektronische Daten] [Meng-Chun Jia, Neelakanta Ravindranath, Vassilios Papadopoulos, Martin Dym] The role of second messenger pathways, cyclic AMP, calcium, and protein kinase C (PKC) in the transcriptional regulation of c-fos protooncogene expression in rat Sertoli cells was investigated. c-fos expression was monitored by Northern blot analysis. Although the action of FSH on Sertoli cells is considered to be mediated by CAMP, dibutyryl CAMP (dbcAMP), a potent membrane permeable analog of cAMP, induced much less c-fos mRNA expression than FSH (<50%) suggesting that additional cAMP-independent mechanisms may mediate the effect of FSH on c-fos. Specific intracellular inhibitors of PKC decreased c-fos induction in response to FSH by more than 50%. lonomycin, which increases intracellular free calcium concentration, induced c-fos expression significantly. These data demonstrate that Sertoli cell c-fos mRNA expression is under multifactorial regulation by CAMP, calcium, and PKC. Kluwer Academic Publishers, 1996 follicle-stimulating hormone nationallicence c-fos nationallicence Sertoli cell nationallicence testis nationallicence Jia Meng-Chun Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut Ravindranath Neelakanta Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut Papadopoulos Vassilios Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut Dym Martin Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 43-49 0300-8177 156:1<43 1996 156 11010 https://doi.org/10.1007/BF00239318 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00239318 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Meng-Chun Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut NATIONALLICENCE 700 1- Ravindranath Neelakanta Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut NATIONALLICENCE 700 1- Papadopoulos Vassilios Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut NATIONALLICENCE 700 1- Dym Martin Department of Cell Biology, Georgetown University Medical Center, 20007, Washington, DC, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/1(1996-03-01), 43-49 0300-8177 156:1<43 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer