caa a22 4500 477118615 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00426334 doi (NATIONALLICENCE)springer-10.1007/BF00426334 Fructose 2,6-bisphosphate metabolism during megakaryocytic differentiation of K562 and MEG-01 cells [Elektronische Daten] [Elisabetta Meacci, Valeria Vasta, Marta Farnararo, Paola Bruni] Fructose 2,6-bisphosphate (Fru-2,6-P2) represents the most powerful activator of 6-phosphofructo-l-kinase, rate-limiting enzyme of glycolysis. Fru-2,6-P2 content is tightly regulated and appears to be under the control of different hormones and growth factors, acting either through covalent modification of isoenzymatic forms of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2), the bifunctional enzyme responsible for the synthesis and the degradation of the compound, or through changes in transcription rate and/or in the expression of different isoforms of the enzyme. In the present study the metabolism of Fru-2,6-P2 was investigated during the differentiation toward megakaryocytes induced by phorbol 12-myristate 13-acetate (PMA) treatment of human leukemia K562 and MEG-O1 cell lines. Fru-2,6-P2 content as well as PFK-2 activity were increased in a dose-dependent manner after 4 days of incubation with PMA. MEG-01 cells resulted more sensitive to the effect of the inducer, anyway in both cell types cytostatic concentrations of the phorbol ester were able to affect Fru-2,6-P2 metabolism. The effect of PMA was maximal at 4 days of incubation in both examined cell lines. Interestingly, the effect induced by the phorbol ester at 4 days was still appreciable subculturing K562 and MEG-01 cells for 3 days in the absence of the inducer and was associated with relevant changes in the molecular properties of PFK-2: namely increased Vmax and Km. This latter finding suggests that the rise in Fru-2,6-P2 content during the differentiation process toward megakaryocytes might result from the expression of a novel PFK-2 isoform. Kluwer Academic Publishers, 1996 fructose 2,6-bisphosphate nationallicence 6-phosphofructo-2-kinase nationallicence K562 cell differentiation nationallicence MEG-01 cell differentiation nationallicence Fru-2,6-P2 : fructose 2,6-bisphosphate nationallicence PFK-2/FBPase-2 : 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (EC 2.7.1.105) nationallicence PMA : phorbol 12-myristate 13-acetate nationallicence Meacci Elisabetta Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut Vasta Valeria Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut Farnararo Marta Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut Bruni Paola Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 125-130 0300-8177 156:2<125 1996 156 11010 https://doi.org/10.1007/BF00426334 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00426334 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meacci Elisabetta Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Vasta Valeria Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Farnararo Marta Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut NATIONALLICENCE 700 1- Bruni Paola Department of Biochemical Sciences, University of Firenze, Viale G.B. Morgagni 50, 50134, Firenze, Italy aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 125-130 0300-8177 156:2<125 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 477118615 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer