caa a22 4500 477118658 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00426339 doi (NATIONALLICENCE)springer-10.1007/BF00426339 Sridhara Rao B. Division of Molecular Virology and Immunology, Georgetown University Medical Center, 5640 Fishers Lane, 20852, Rockville, MD, USA aut Regulation of DNA replication by homopurine/homopyrimidine sequences [Elektronische Daten] [B. Sridhara Rao] The simple repeating homopurine/homopyrimidine sequences dispersed throughout many eukaryotic genomes are known to form triple helical structures comprising three-stranded and single-stranded DNA. Several lines of evidence suggest that these structures influence DNA replication in cells. Homopurine/homopyrimidine sequences cloned into simian virus 40 (SV40) or SV40 origin-containing plasmids caused a reduced rate of DNA synthesis due to the pausing of replication forks. More prominent arrests were observed in in vitro experiments using single-stranded and double-stranded DNA with triplex-forming sequences. Nucleotides unable to form triplexes when present in the template DNA or when incorporated into the nascent strand prevented termination. Similarly, mutations destroying the triplex potential did not cause arrest while compensatory mutations restoring triplex potential restored it. These and other observations from a number of laboratories indicating that homopurine/homopyrimidine sequences act as arrest signals in vitro and as pause sites in vivo during replication fork movement suggest that these naturally occurring sequences play a regulatory role in DNA replication and gene amplification. Kluwer Academic Publishers, 1996 DNA replication nationallicence homopurine nationallicence homopyrimidine nationallicence triplex nationallicence pausing nationallicence arrest nationallicence Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 163-168 0300-8177 156:2<163 1996 156 11010 https://doi.org/10.1007/BF00426339 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00426339 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sridhara Rao B. Division of Molecular Virology and Immunology, Georgetown University Medical Center, 5640 Fishers Lane, 20852, Rockville, MD, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 163-168 0300-8177 156:2<163 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer