caa a22 4500 477118690 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00426332 doi (NATIONALLICENCE)springer-10.1007/BF00426332 Receptor-mediated endocytosis of fucosylated neoglycoprotein by macrophages [Elektronische Daten] [Kakali arkar, Himadri Sekhar Sarkar, Labanyamoy Kole, Pijush Das] The characteristics of the recognition system involved in the receptor mediated endocytosis of the neoglycoprotein, fucose human serum albumin (HSA) were studied. It was found that (i) fucose-HSA showed strong affinity binding and uptake by various macrophages; (ii) binding was specific for L-fucose and D-mannose; (iii) binding was found to be inhibited by oxidant like H2O2 and swainsonine whereas it was elevated by dexamethasone; (iv) clearance of125I-fucose-HSA was rapid and strongly inhibited by unlabelled fucose-HSA. Greater than 70% of fucose-HSA was found in liver and more than 60% of this was found in liver lysosomes; (v) uptake of fucose-HSA was thirty-fold more efficient in liver macrophages (Kupffer cells) than in hepatocytes; (vi) moreover, mannose-HSA and ovalbumin which are potent inhibitors of mannose/N-acetylglucosamine receptors inhibited clearance and uptake of fucose-HSA by liver as well as by isolated Kupffer cells suggesting the involvement of both fucose and mannose receptors or a single type of receptor having greater affinity for fucose-HSA than for mannose-HSA. These results emphasize the important role of fucose-terminated glycoproteins in site-specific drug targeting. Kluwer Academic Publishers, 1996 macrophages nationallicence neoglycoproteins nationallicence sugar receptors nationallicence endocytosis nationallicence arkar Kakali Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut Sekhar Sarkar Himadri Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut Kole Labanyamoy Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut Das Pijush Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 109-116 0300-8177 156:2<109 1996 156 11010 https://doi.org/10.1007/BF00426332 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00426332 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- arkar Kakali Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut NATIONALLICENCE 700 1- Sekhar Sarkar Himadri Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut NATIONALLICENCE 700 1- Kole Labanyamoy Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut NATIONALLICENCE 700 1- Das Pijush Department of Molecular Cell Biology, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, 700032, Calcutta, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 109-116 0300-8177 156:2<109 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer