caa a22 4500 477118712 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00426337 doi (NATIONALLICENCE)springer-10.1007/BF00426337 Short chain fatty acid and glucose metabolism in isolated pig colonocytes: modulation by NH4+ [Elektronische Daten] [Béatrice Darcy-Vrillon, Claire Cherbuy, Marie-Thérèse Morel, Michelle Durand, Pierre-Henri Duée] Short chain fatty acids (SCFA) from bacterial origin, as well as glucose from vascular origin, are among fuel substrates available to the colonic mucosa. The present work investigated the possible modulation by another bacterial metabolite, i.e. ammonia, of the capacities of colonic epithelial cells to metabolize these substrates. Viable colonocytes were isolated from the proximal colon of 40-50 kg pigs fed a standard diet and were incubated (30 min, 37°C) in the presence of a concentration range of 14C-labeled n-butyrate or acetate, or 14C-labeled glucose (5 mm), with or without NH4Cl (10 mM) addition. 14CO2 and metabolites generated were measured. Butyrate utilization resulted in a high generation of ketone bodies (acetoacetate and β-OH-butyrate), in addition to 14CO2 production. However, the net ketone body generation was significantly decreased for butyrate concentrations higher than 10 mM. In contrast to n-butyrate, acetate when given as the sole substrate got preferentially metabolized in the oxidation pathway. Acetate metabolism was not affected by NH4Cl, thus indicating that the tricarboxylic acid cycle was unchanged. Conversely, 4C02 and ketone body production from butyrate were decreased by 30% in the presence of NH4Cl, suggesting that butyrate activation or β-oxidation was diminished. Glucose utilization rate was increased by 20%, due to an increased glycolytic capacity in the presence of NH4Cl. A dose-dependent stimulation of phosphofructokinase activity by NH4 + could account for this effect. It is concluded that ammonia, whose physiological concentration is high in the colonic lumen, can modulate the metabolism of two major substrates, n-butyrate and glucose, in colonic epithelial cells. Kluwer Academic Publishers, 1996 pig nationallicence colonocytes nationallicence metabolism nationallicence short-chain fatty acids nationallicence glucose nationallicence NH4+ nationallicence Darcy-Vrillon Béatrice Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut Cherbuy Claire Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut Morel Marie-Thérèse Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut Durand Michelle Unité de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut Duée Pierre-Henri Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 145-151 0300-8177 156:2<145 1996 156 11010 https://doi.org/10.1007/BF00426337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00426337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Darcy-Vrillon Béatrice Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut NATIONALLICENCE 700 1- Cherbuy Claire Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut NATIONALLICENCE 700 1- Morel Marie-Thérèse Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut NATIONALLICENCE 700 1- Durand Michelle Unité de Nutrition et Sécurité Alimentaire, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut NATIONALLICENCE 700 1- Duée Pierre-Henri Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, F-78352, Jouy-en-Josas, Cedex, France aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers 156/2(1996-09-01), 145-151 0300-8177 156:2<145 1996 156 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer