caa a22 4500 477118747 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250994 doi (NATIONALLICENCE)springer-10.1007/BF00250994 Hierarchies in the binding of human factor XIII, factor XIIIa, and endothelial cell transglutaminase to human plasma fibrinogen, fibrin, and fibronectin [Elektronische Daten] [Komandoor Achyuthan, Tami Rowland, Paul Birckbichler, Kyung Lee, Paul Bishop, Ann Achyuthan] The affinities of Factor XIII (FXIII), Factor XIIIa (FXIIla), and cellular transglutaminase (Tg) for fibrinogen (Fgn), fibrin (Fbn), and fibronectin (Fn) were compared using a solid-phase binding assay. Initial rates of binding were as follows: FXIII bound Fbn 3-fold more than Fgn. FXIII did not bind Fn till 20 min. Increasing the ligands concentrations and binding time, resulted in weak binding of FXIII to Fn. FXIIla bound Fbn 2-fold more than Fgn and 28-fold more than Fn. Tg bound Fn ∼ 130-fold more than either Fgn or Fbn. At equilibrium, the extent of binding was determined to be as follows: FXIII bound Fbn 3-15-fold more than Fgn and 8-fold more than Fn. FXIIIa bound Fgn and Fbn equally and 12-25-fold more than Fn. FXIIla bound Fgn or Fbn 2-fold and 25-fold greater than FXIII-Fbn and FXIII-Fgn interactions, respectively. Tg bound about equally to Fgn and Fbn and 10-20-fold less than Fn. The K d s′ for FXIIla binding to Fn, Fgn, and Fbn were 100, 23, and 19 nM, respectively. The K d for Tg binding to Fn was 6.5 nM. The binding hierarchies are: [Tg-Fn]>[FXIIIa-Fgn]=[FXIIIa-Fbn]>[FXIII-Fbn]>[FXIIIFgn]=[FXIIIa-Fn]>[Tg-Fbn]=[Tg-Fgn]>[FXIII-Fn]. Such hierarchies could regulate the cross-linkings by FXIIIa and Tg during hemostasis, wound healing, and cell adhesion. Kluwer Academic Publishers, 1996 transglutaminase nationallicence factor XIII nationallicence fibrinogen nationallicence fibrin nationallicence fibronectin nationallicence binding nationallicence Tg : cellular transglutaminase nationallicence FXIII : coagulation factor XIII nationallicence FXIIla : factor XIIIa (thrombin-activated FXIII) nationallicence Fgn : human plasma fibrinogen nationallicence Fn : human plasma fibronectin nationallicence Fbn : human plasma fibrin (thrombin-cleaved fibrinogen) nationallicence ECM : extracellular matrix nationallicence Achyuthan Komandoor Oklahoma Medical Research Foundation, ZymeTx, Inc., 73104, Oklahoma City, OK aut Rowland Tami Departments of Urology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut Birckbichler Paul Departments of Urology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut Lee Kyung Departments of Medicine, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut Bishop Paul Zymogenetics Inc., 98102, Seattle, WA, USA aut Achyuthan Ann Departments of Biochemistry & Molecular Biology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 43-49 0300-8177 162:1<43 1996 162 11010 https://doi.org/10.1007/BF00250994 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250994 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Achyuthan Komandoor Oklahoma Medical Research Foundation, ZymeTx, Inc., 73104, Oklahoma City, OK aut NATIONALLICENCE 700 1- Rowland Tami Departments of Urology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut NATIONALLICENCE 700 1- Birckbichler Paul Departments of Urology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut NATIONALLICENCE 700 1- Lee Kyung Departments of Medicine, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut NATIONALLICENCE 700 1- Bishop Paul Zymogenetics Inc., 98102, Seattle, WA, USA aut NATIONALLICENCE 700 1- Achyuthan Ann Departments of Biochemistry & Molecular Biology, Oklahoma University Health Sciences Center, 73104, Oklahoma City, OK aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 43-49 0300-8177 162:1<43 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer