caa a22 4500 477118755 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250993 doi (NATIONALLICENCE)springer-10.1007/BF00250993 Anatomical distribution of NADPH-cytochrome P450 reductase and cytochrome P4502D forms in rat brain: Effects of xenobiotics and sex steroids [Elektronische Daten] [Arthur Berghl, Henry Strobel] Since the brain is not a homogeneous organ, but one dependent upon the well orchestrated interaction of numerous parts, pathology in one nucleus may have a large impact upon its overall function. Hence, the anatomical distribution of the P450 monoxygenase system in brain, as well as the regulation of its expression, is important in elucidating its function in that organ. In order to study these issues, female rats-both ovariectomized and not-were treated with a number of xenobiotic compounds and sex steroids. The brains from these animals were dissected into 8 discrete regions and the presence and relative level of message for P4502D and P450 reductase determined using polymerase chain reaction. Results of this investigation indicate the presence of mRNA for reductase and P4502D isoforms throughout the rat brain. In addition, quantitative PCR was utilized to demonstrate the effects of xenobiotics (phenobarbital, β-naphthoflavone, imipramine) and sex hormones (testosterone, estrogen) on the levels of these messages in the female rat brain. Significant induction of message for P4502D forms was noted with testosterone in the absence of estrogen. The level of mRNA for reductase was not significantly influenced by any of the treatments, however. These results raise the issue of a sexual dimorphism in the rat regarding P4502D expression in brain. Kluwer Academic Publishers, 1996 cytochrome P450 nationallicence brain nationallicence sex steroids nationallicence competitive PCR nationallicence RT : reverse transcription nationallicence PB : phenobarbital nationallicence βNF : β-naphthoflavone nationallicence ovx : ovariectomized nationallicence reductase : cytochrome P450 reductase nationallicence P450 : cytochrome P450 nationallicence Berghl Arthur Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas, USA aut Strobel Henry Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 31-41 0300-8177 162:1<31 1996 162 11010 https://doi.org/10.1007/BF00250993 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250993 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Berghl Arthur Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas, USA aut NATIONALLICENCE 700 1- Strobel Henry Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 31-41 0300-8177 162:1<31 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer