caa a22 4500 477118763 CHVBK 20180405111629.0 cr unu---uuuuu 170330e19960301xx s 000 0 eng 10.1007/BF00250990 doi (NATIONALLICENCE)springer-10.1007/BF00250990 Effect of elevated glucose concentrations on cellular lipid peroxidation and growth of cultured human kidney proximal tubule cells [Elektronische Daten] [Sushil Jain, Khandoker Morshed, Krishnaswamy Kannan, Kenneth McMartin, Joseph Bocchini Jr.] This study has examined whether elevated glucose can induce lipid peroxidation and contribute to the inhibition of cell growth in human kidney proximal tubule(HPT) cells. HPT cells were cultured in media containing glucose concentrations of 8 mM (control), 25 mM, and 50 mM. Lipid peroxidation was assessed by the thiobarbituric acid reactivity and cell growth was assessed by 3H-thymidine uptake. Results show decreased (59%, p < 0.01) growth of HPT cells cultured in 50 mM glucose. Cells cultured in 50 mM mannitol did not show any growth inhibition, suggesting that the decreased cell growth associated with glucose is not due to osmolarity changes. There was an increase (108%, p < 0.02) in lipid peroxidation in cells cultured with high levels of glucose (50 mM) compared with controls and cells cultured with 50 mM mannitol. To examine if membrane lipid peroxidation or malondialdehyde (MDA, an end product of lipid peroxidation) has any role in the inhibition of cell growth, we examined the effect of tertiary butylhydroperoxide (TBH, known to cause lipid peroxidation and generate MDA) on the growth of HPT cells. TBH decreased cell growth (49, 17 and 3% of controls at 0.1, 0.25, and 0.5 [mole TBH/ml medium). Similarly, a marked reduction in the growth was observed with exogenous MDA (72, 69 and 34% of controls at 0.1, 0.25, and 0.5 μmole MDA/ml medium). This suggests that elevated glucose can induce membrane lipid peroxidation and accumulation of MDA, which in turn can inhibit cellular growth and contribute to the altered structure and function of HPT cells in diabetes. Kluwer Academic Publishers, 1996 diabetes nationallicence lipid peroxidation nationallicence free radicals nationallicence human proximal tubule cells nationallicence cell growth nationallicence Jain Sushil Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut Morshed Khandoker Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut Kannan Krishnaswamy Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut McMartin Kenneth Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut Bocchini Jr. Joseph Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 11-16 0300-8177 162:1<11 1996 162 11010 https://doi.org/10.1007/BF00250990 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00250990 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jain Sushil Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut NATIONALLICENCE 700 1- Morshed Khandoker Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut NATIONALLICENCE 700 1- Kannan Krishnaswamy Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut NATIONALLICENCE 700 1- McMartin Kenneth Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut NATIONALLICENCE 700 1- Bocchini Jr Joseph Departments of Pediatrics and Pharmacology, Louisiana State University School of Medicine, 71130, Shreveport, LA, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Kluwer Academic Publishers; http://www/wkap.nl 162/1(1996-03-01), 11-16 0300-8177 162:1<11 1996 162 11010 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer